维生素B_1、B_(12)在雨生红球藻不同培养阶段的作用研究  

The Effect of Vitamin B_1,B_(12) on the Two Culture Stage of Haematococcus plvialis

作　　者：张英[1] 蔡明刚[1] 齐安翔[1] 金贵娥[1] 王鑫煌[1] 

机构地区：[1]厦门大学海洋与环境学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2004年第B08期142-146,共5页Journal of Xiamen University：Natural Science

基　　金：厦门市科技项目(3052Z20031086);海洋与环境学院学生科研创新项目(2003);"挑战杯"科研计划大赛厦门大学学生课外科研自强启动基金(2004)联合资助

摘　　要：研究了维生素B1、B12对雨生红球藻营养生长及胁迫阶段各生长指标的影响.研究结果表明适量维生素的加入具有促进雨生红球藻生长、提高胁迫条件下的微藻存活率、以及促进虾青素积累等作用.其中,在培养基中添加w(B12)=5×10-8维生素B12,可以使雨生红球藻的生长率、存活率和虾青素产量分别较空白组提高21%、264%和43%以上.此外,研究还发现在单独加入维生素B1和B12的培养组中,大部分培养组的孢子比率均明显下降,这为降低虾青素的提取难度和提高虾青素的利用率提供了新的思路.The effects of Vitamin B_1 and Vitamin B_(12) on the two culture stages of Haematococcus plvialis were studied.The results showed that the growth rate,the survival rate,the static cell rate and the astaxanthin production under stress condition were improved when the media(BBM) was enriched with Vitamin B_1 and Vitamin B_(12).Especially when w(B_(12)) =5×10^(-8)of Vitamin B_(12) was added in the traditional media the growth rate,the survival rate and the astaxanthin production were enhanced with over 21%、264% and 43% higher than the controls,respectively.Additionally,the static cell ratio can be mostly reduced by added the Vitamin B_1 and B_(12) separately,which made the efficiency of astaxanthin extraction heighten.

关 键 词：维生素B1 维生素B12 雨生红球藻 虾青素 养殖 营养细胞 

分 类 号：S968.41[农业科学—水产养殖]