胃癌和相应癌旁组织中细胞凋亡与bcl-2的表达  

APOPTOSIS AND EXPRESSION OF bcl-2 IN GASTRIC CARCINOMA AND CORRESPONDING ADJACENT TISSUES

作　　者：时飞[1] 阎丽平[2] 姜晓华[3] 李莉[4] 

机构地区：[1]青岛市市立医院麻醉科,山东青岛266011 [2]青岛大学医学院微生物学教研室 [3]中国人民解放军第107医院 [4]青岛市市立医院检验科

出　　处：《齐鲁医学杂志》2004年第6期491-492,共2页Medical Journal of Qilu

摘　　要：①目的　探讨细胞凋亡和bcl 2蛋白表达在胃癌发生发展中的作用。②方法　选取 5 8例胃癌和相应癌旁组织作为研究对象 ,采用脱氧核苷酸移换酶 (TdT)介导的dUTP缺口末端标记法 (TUNEL)及免疫组化技术检测其细胞凋亡指数 (AI)和bcl 2蛋白的表达。③结果　胃癌和相应癌旁组织中AI及bcl 2蛋白表达差异有显著性 (t=13.4 0 0 ,P <0 .0 1;χ2 =4 .32 1,P <0 .0 5 )。bcl 2表达阳性组AI明显低于bcl 2表达阴性组 ,差异有显著性 (t=2 .381,P <0 .0 5 )。④结论　bcl 2蛋白异常表达可抑制细胞凋亡 。Objective To explore the roles of apoptosis and bcl-2 protein expression in the oncogenesis and development of gastric carcinoma. Methods The apoptotic index(AI) and the expression of bcl-2 protein were tested by terminal deoxynucleotidy1 transferase(TdT)-mediated dUTP-biotin nick end labelling(TUNEL) and immunohistochemistry in 58 gastric carcinoma and corresponding adjacent tissues. Results There was a significant difference in AI and the expression of bcl-2 protein between carcinoma and the corresponding adjacent tissues (t=13.400,P<0.01;χ 2=4.321,P<0.05). The apoptotic cells was significantly fewer in bcl-2-postive tumors than in bcl-2-negative ones (t=2.381,P<0.05). Conclusion The abnormal expression of bcl-2 is involved in the apoptosis and correlates with oncogenesis of gastric carcinomas.

关 键 词：胃肿瘤 细胞凋亡 蛋白质BCL-2 

分 类 号：R735.2[医药卫生—肿瘤]