癌症恶病质与细胞因子的关系及消炎痛干预的实验研究  

Relationship of cytokine level with cancer cachexia and therapeutic effects of indomethacin for cancer cachexia

作　　者：徐建芳[1] 周彩存[1] 张海平[1] 粟波[1] 唐亮[1] 成倍玲[1] 秦萍[1] 李爱武[1] 

机构地区：[1]上海市肺科医院肿瘤科,200433

出　　处：《中国肺癌杂志》2004年第4期336-338,共3页Chinese Journal of Lung Cancer

摘　　要：目的　探讨癌症恶病质实验模型中细胞因子IL 1、IL 6、TNF α、IFN γ水平的变化 ,观察常规剂量消炎痛对癌症恶病质的改善效果以及对小鼠生存时间的影响。方法　用Lewis肺癌细胞株接种纯种C57小鼠建立癌症恶病质的实验模型 ,检测小鼠在不同阶段血清IL 1、IL 6、TNF α、IFN γ的水平及体重变化 ,同时观察每日腹腔内注射常规剂量消炎痛对癌症恶病质的治疗疗效。结果　癌症恶病质组小鼠血清IL 1、IL 6、TNF α的水平显著高于健康对照组 (P <0 .0 5 ) ,而体重明显低于健康对照组 (P <0 .0 5 )。癌症恶病质小鼠中 ,消炎痛治疗组小鼠的体重明显高于生理盐水对照组且生存时间延长 (P <0 .0 5 ) ,而血清IL 1、IL 6、TNF α的水平显著低于生理盐水对照组 (P <0 .0 5 )。血清IFN γ在癌症恶病质小鼠与健康对照组之间 ,在消炎痛治疗组与生理盐水对照组之间比较差异均无显著性 (P >0 .0 5 )。结论　IL 1、IL 6、TNF α可能参与癌症恶病质的发生发展 ,通过采用消炎痛积极干预 ,能明显降低血清IL 1、IL 6、TNF α的水平 ,改善恶病质 ,延长生存期。IFN γ不参与癌症恶病质的发生发展。Objective To investigate the relationship between cytokine level and cancer cachexia on murine model, and to observe the effects of regular dose of indomethacin on cancer cachexia and survival of mice. Methods C57 mice bearing Lewis lung carcinoma were used to establish murine cancer cachexia model. The serum cytokine levels (IL 1, IL 6, TNF α and IFN γ) and body weight of the mice were measured at different time points before and after treatment with daily intraperioneal injection of either indomethacin (1 mg/kg) or saline. Results The mice in cancer cachexia group had significantly higher serum levels of IL 1, IL 6 and TNF α ( P <0.05) and lower body weight ( P <0.05) than those in healthy control group. Compared to saline treatment, indomethacin intervention apparently down regulated the levels of IL 1, IL 6 and TNF α ( P < 0.05 ), and remarkably prolonged the survival of mice ( P <0.05). No significant difference in IFN γ level was observed between cancer cachexia and healthy control groups ( P >0.05), as well as between indomethacin and saline groups ( P >0.05). Conclusion The results suggest that serum IL 1, IL 6 and TNF α are possibly associated with cancer cachexia, however, IFN γ seems to be irrelevant. Indomethacin may potentially ameliorate cancer cachexia through down regulating levels of cytokines.

关 键 词：血清 IL-1 IL-6 TNF-Α IFN-Γ 癌症恶病质 消炎痛 

分 类 号：R73-3[医药卫生—肿瘤]