溶脲脲原体感染对精卵结合相关分子硫酸半乳糖甘油酯的影响  

Influence of Ureaplasma Urealyticum Infection on the Sperm-egg Binding Associated Molecule, Sulfogalactosylglycerolipid

作　　者：武婷婷[1] 鲁梅格[1] 胡燕琴[1] 郭强苏[1] 徐晨[1] 

机构地区：[1]上海第二医科大学组织胚胎学教研室,上海200025

出　　处：《中华男科学杂志》2004年第9期651-654,共4页National Journal of Andrology

基　　金：国家自然科学基金 ( 3 9870 3 74);973课题 (NO2 0 0 1CB5 10 3 0 5 );上海市教委曙光计划基金 ( 99SG42 )资助

摘　　要：目的 :研究溶脲脲原体 (Uu)感染对精卵结合相关分子硫酸半乳糖甘油酯 (SGG)的影响。　方法 :取成熟小鼠附睾精子制成精子悬液 ,随机分为 4组 :Uu组 (与Uu悬液共孵育 )、培养液组 (与Uu培养液共孵育 )、正常组和免疫前血清 (PRS)组。间接免疫荧光法检测SGG在精子膜表面的定位以及Uu对精子膜表面SGG的影响。　结果 :SGG主要位于附睾精子头部相当于顶体区的质膜上。与Uu培养液共孵育的精子 ,其表面SGG的阳性率达82 .0 % ;而与Uu悬液共孵育的精子 ,其表面SGG的阳性率下降至 39.0 % (P =0 .0 0 1)。　结论 :Uu能与精子膜SGG结合 ,SGG可能是Uu在哺乳动物精子膜上的受体 ,Uu封闭精卵结合相关分子SGG可能是Uu感染引起男性不育的分子机制之一。Objective: To study the influence of Ureaplasma urealyticum (Uu) infection on the sperm-egg binding associated molecule, sulfogalactosylglycerolipid(SGG). Methods: Epididymal sperm was collected from adult mice. The sperm suspension was randomly divided into 4 groups: Uu group (coincubated with Uu suspension), medium group (coincubated with Uu medium), normal group and PRS group. The indirect immunofluorescence technique was used to localize SGG on the sperm membrane and to observe the influence of Uu on SGG. Results: In the epididymal sperm, SGG was localized to the head plasma membrane overlaying the acrosomal region. The SGG-positive rate of the sperm coincubated with Uu medium was 82.0%, while that of the sperm coincubated with Uu suspension was reduced to 39.0% (P= 0.001). Conclusion: Uu can adhere to the sperm surface. SGG might be a membrane receptor on the sperm surface for Uu infection of the mammalian male genital tract. The blockage of SGG by Uu might be one of the molecular mechanisms correlative to male infertility induced by Uu infection.

关 键 词：男性不育 溶脲脲原体 硫酸半乳糖甘油酯 精卵结合 

分 类 号：R363[医药卫生—病理学]