抗生素S_(632)A_3在体外抗柯萨奇病毒B_(3m)株作用的研究  

Action of Antibiotics S_(632) A_3 on Virus COX B_(3m) in vitro

作　　者：林睿[1] 荀培[1] 张媛媛[1] 石立莹[2] 李梅[2] 何健民[2] 李晓眠[2] 

机构地区：[1]天津医科大学临床医学七年制,天津300070 [2]天津医科大学微生物教研室,天津300070

出　　处：《天津医科大学学报》2004年第3期374-375,399,共3页Journal of Tianjin Medical University

摘　　要：目的 :体外测定抗生素S632A3 抗柯萨奇病毒B3m 株的作用 ,为筛选新型抗病毒药物提供参考依据。方法 :用不同稀释度的抗生素S632A3 抑制柯萨奇病毒B3m 株对Vero细胞的感染作用 ,48h后观察细胞病变效应 ,并用MTT法测定细胞活性。结果 :抗生素S632A3 能明显抑制柯萨奇病毒对Vero细胞的致病变作用 ,使细胞存活率升高。结论 :该药物有较显著的抗柯萨奇病毒B3m 株的作用 ,且对细胞的毒性低 ,但该药物对柯萨奇病毒B3m 株无预防作用 ,是一种具有潜在开发前景的药物。Objective:To Study the action of Antibiotics S 632 A 3 on Virus COX B 3m in vitro,to provide reference gist for filtrating new antivirus drug.Methods:Diluted the Antibiotics S 632 A 3 to different concentrations,and infecting the Vero cell.After48h the cell's pathological changes was observed,and the cell's activity was tested by MTT.Results:Antibiotics S 632 A 3 conld inhibit Virus COX's pathological effect on Vero cell,and raise the cell's libability.Conˉclusion:This drug has marked effect on resisting Virus COX B 3m ,and it's toxicity to cells is low,but this drug has not preventive effect on Virus COX B 3m ,it has potential exploring future.

关 键 词：抗生素8632A3 柯萨奇病毒B3m株 VERO细胞 MTT 

分 类 号：R373.23[医药卫生—病原生物学]