尼美舒利对人胃癌细胞株SGC-7901增殖和凋亡的影响  

Effects of nimesulide on proliferation and apoptosis of human gastric carcinoma cells SGC-7901

作　　者：谯敏[1] 高青[1] 王丕龙[1] 张俊文[1] 

机构地区：[1]重庆医科大学临床学院消化内科,重庆400016

出　　处：《重庆医科大学学报》2004年第5期654-656,660,共4页Journal of Chongqing Medical University

摘　　要：目的 :体外研究尼美舒利对人胃癌细胞株SGC - 790 1增殖的影响 ,并探讨其可能的作用机制。方法 :采用MTT法、流式细胞仪 (FCM)、电镜等技术 ,体外研究尼美舒利对人胃癌细胞株SGC - 790 1增殖和影响及可能的机制。结果 :MTT显示尼美舒利 (12 .5～ 4 0 0 μmol/L)呈时间—剂量依赖方式抑制人胃癌细胞株SGC - 790 1的增殖。FCM显示 ,DNA直方图上出现典型的亚二倍体“凋亡峰”。S期、G2 /M期细胞比例升高 ,G1期细胞比例下降 ,阻止细胞周期的进展。电镜下见典型的细胞凋亡形态特征 :细胞核染色质致密浓缩 ,凋亡小体形成等。结论 :尼美舒利体外能显著抑制人胃癌细胞株SGC - 790 1生长 ,可能与诱导细胞凋亡阻止细胞周期进展有关。Objective:To study the effects of nimesulide on proliferation and apoptosis of human gastric carcinoma cells SGC-7901 in vitro,and provide an experimental basis for clinical application.Methods:Proliferation of human gastric carcinoma cells SGC-7901 was assessed by MTT,and apoptosis was detected by transmission electron microscope and flow cytometry.Results:Proliferation of SGC-7901 was inhibited significantly by nimesulide(range from 12.5 to 800μmol/L) in a dosage-dependant manner ( P <0.05).Under transmission electron microscope and fluorescent microscope,some SGC-7901 cells undergo a typical apoptosis after 4 days incubation with nimesulide.There is an apoptosis peak.Conclusion:Nimesulide can suppress proliferation of SGC-7901 cells,which may be related to the mechanism of inducing cell apoptosis and preventing development of cell cycle.

关 键 词：人胃癌细胞株SGC-7901 尼美舒利 细胞凋亡 增殖 

分 类 号：R735.2[医药卫生—肿瘤]