单侧坐骨神经完全结扎术后Ⅰ型囊泡膜谷氨酸转运体在大鼠腰髓、背根神经节和结扎远侧端神经干内表达的变化(英文)  

Change of expression of vesicular glutamate transporter 1 in the lumbar spinal cord,dorsal root ganglia and nerve stump distal to ligation after complete ligation of the unilateral sciatic nerve

作　　者：屈金良[1] 刘涛[1] 庞有旺[1] 李金莲[1] 

机构地区：[1]第四军医大学基础医学部解剖学教研室梁銶锯脑研究中心,陕西西安710032

出　　处：《中国神经科学杂志》2004年第5期331-335,共5页

基　　金：第四军医大学骨干人才资助计划资助

摘　　要：目的　 研究单侧坐骨神经结扎对大鼠腰 4～ 5脊髓节段和相应的背根神经节 (DRGs)内VGluT1样免疫阳性反应产物表达的影响以及VGluT1通过轴浆流向外周转运的情况。 方法　 采用免疫组织化学方法观察单侧坐骨神经结扎后不同时间内腰 4～ 5脊髓节段、DRGs和结扎部位的近、远侧端神经干内VGLuT1样免疫阳性反应强度的变化。结果　 (1)坐骨神经结扎后第 1和第 2天 ,VGluT1样免疫阳性产物在结扎的同侧腰 4～ 5脊髓节段和相应节段的DRGs内未检测到明显变化 ;但自术后第 4天开始 ,可观察到VGluT1样免疫阳性产物的表达在上述部位逐渐减弱 ;VGluT1样免疫阳性产物表达的降低在上述部位所出现的时间和程度相平行。 (2 )结扎后第 1天即可观察到VGluT1样免疫阳性产物在坐骨神经结扎部位近侧端的表达有所升高 ,但自术后第 4天开始逐渐降低 ;而VGluT1样免疫阳性产物在坐骨神经结扎部位远侧端的表达自结扎后第 1天起就逐渐降低 ,至第 4周时已完全消失。结论　 (1)DRG神经元合成VGluT1,并通过轴浆流将VGLluT1向中枢突和周围突运输 ,故腰髓内部分VGluT1样阳性末梢起源于DRG神经元 ;(2 )Objective To study the effects of complete ligation of the unilateral sciatic nerve (SN) on the expression of VGluT1-like immunoreactivity (VGluT1-LI) in the fourth and fifth lumbar spinal cord (L4, L5) , corresponding dorsal root ganglia (DRGs) and transport of VGluT1-LI by axonal flow peripherally through peripheral axons of DRG neurons. Method Immunohistochemistry was used to examine the change of VGluT1-LI in the L4 and L5, corresponding DRGs and the nerve stump proximal or distal to ligation in the different times after unilateral ligation of the SN. Results (1) Obvious change of VGluT1-LI in the neuronal terminals of ipsilateral L4, L5 and cell bodies of DRGs was not detected in the first 2 d after complete ligation of the unilateral SN. But from the 4th day (4 d) after ligation, a persistent decrease of VGluT1-LI was shown in the neuronal terminals ipsilateral L4, L5 and corresponding DRG neurons. The reduction of VGluT1-LI in the spinal cord (SC) was paralleled with that in DRG neurons in the time and degree;(2) VGluT1-LI was observed to increase in the nerve stump proximal to ligation 1d after unilateral ligation of the SN, but to decrease persistently in the nerve stump proximal to ligation from 4 d. On the other hand, VGluT1-LI decreased persistently in the nerve stump distal to ligation from 1 d and disappeared from 4 d after unilateral ligation of the SN. Conclusion (1) VGluT1 was synthesized in cell bodies of DRG neurons and was transported centrally and peripherally by axonal flow. Therefore, part of the VGluT1 in the SC originated from DRG neurons;(2) VGluT1 synthesis in the DRGs was vulnearable to damage of the peripheral axons.

关 键 词：坐骨神经完全结扎术 Ⅰ型囊泡膜谷氨酸转运体 大鼠 腰髓 背根神经节 神经干内表达 免疫组织化学 

分 类 号：R363[医药卫生—病理学]