白腐真菌漆酶的固定化及其应用研究  

Immobilization of Fungal Laccase on Nylon Net and Application of the Immobilized Enzyme

作　　者：张书祥[1] 肖亚中[1] 王怡平[1] 张敏[1] 

机构地区：[1]安徽大学生命科学学院,合肥230039

出　　处：《微生物学通报》2004年第5期85-88,共4页Microbiology China

基　　金：国家自然科学基金资助项目 (No 3 0 3 70 0 45 );安徽省优秀青年基金资助项目 (No 0 40 43 0 48);安徽省教育厅重点科研项目 (No 2 0 0 2KJ0 3 4zd)

摘　　要：以尼龙网为载体 ,戊二醛为交联剂 ,进行固定化真菌漆酶的条件优化和性质研究 ,优化条件为 :尼龙网在 5 %的戊二醛溶液中交联 6h后 ,加入 30U漆酶溶液固定 8h ,酶活回收率为 5 0 3%。与游离酶相比 ,固定化漆酶的热稳定性明显提高 ,最适pH值略有下降。用该固定化漆酶处理低浓度造纸废水 ,经过 8批次连续试验 ,酶活保留 5 2 %。Condition optimization for immobilization of Fungal laccase with Nylon Net and glutaraldehyde and the nature of the immobilized enzyme were studied. The optimum conditions of the immobilization are: Nylon Net is crosslinked with 5% glutaraldehyde 15mL for 6 hours; the 30U laccase is added for immobilization for 8 hours. On this case, the recovery of enzyme activity was 50.3%. Compared with free enzyme, the thermal stability of immobilized enzyme was improved evidently but the optimal pH decreased slightly. 52% enzyme activity of immobilized laccase was hold after 8 cycles treatment with low concentration pulp wastewater.

关 键 词：尼龙网 固定化 真菌漆酶 

分 类 号：TQ033[化学工程]