口服免疫多糖(酵母细胞壁)对南美白对虾抗哈维弧菌感染的作用  

Influence of Immunopolysaccharide (Yeast Cell Wall)on Resistance to Vibrio harveyi of Penaeus vannamei by Oral Administration

作　　者：陈昌福[1] 姚鹃[2] 陈萱[3] 李兆文[2] 陈超然[1] 梁运祥[4] 

机构地区：[1]华中农业大学水产学院,武汉430070 [2]安琪酵母股份有限公司,宜昌443003 [3]华中农业大学生命科学技术学院,武汉430070 [4]华中农业大学农业微生物学国家重点开放实验室,武汉430070

出　　处：《安徽农业大学学报》2004年第4期402-405,共4页Journal of Anhui Agricultural University

基　　金：国家自然科学基金项目(30271032)资助。

摘　　要：在南美白对虾(Penaeusvannamei)饲料中分别加100 0、200 0、300 0、400 0和500 0mg·kg-1Bm的免疫多糖(酵母细胞壁),连续投喂28d后,通过检测供试虾血清、肝胰腺和肌肉中酸性磷酸酶(ACP)、碱性磷酸酶(ALP)和过氧化物酶(POD)的活性及经过哈维弧菌的活菌攻毒,探讨口服不同剂量的免疫多糖(酵母细胞壁)对南美白对虾体内免疫相关酶活性的影响和对人工感染哈维弧菌抗病力的作用。结果表明,在南美白对虾饲料中添加100 0mg·kg-1Bm的免疫多糖(酵母细胞壁),能显著提高供试南美白对虾血清和肌肉中ACP和ALP活性(t测验,P<0 05),极显著地提高肝胰腺中ACP、ALP和POD的活性(t测验,P<0 01),增强抗哈维弧菌感染的能力。免疫多糖(酵母细胞壁)不同添加剂量的试验组之间则未出现显著差别(t测验,P>0 05)。试验结果表明免疫多糖(酵母细胞壁)在南美白对虾饲料中的添加量以100 0mg·kg-1Bm为宜。The pacific white Shrimp,Penaeus vannamei were fed with feed supplemented with immunopolysaccharied(yeast cell wall)at the ratio of 100.0,200.0,300.0,400.0 and 500.0 mg·kg^(-1)Bm,respectively. 28 days later, in order to find out the effect of yeast cell wall of different ratios on immuno-related enzyme activities in Penaeus vannamei as well as their resistance to Vibrio harveyi,the acid phosphatase(ACP), alkaline phosphatase(ALP)and peroxidase (POD)in the serum, hepatopancreas and muscle were monitored respectively. Meanwhile, the Penaeus vannamei were challenged with live Vibrio harveyi.The results showed that when fed with feed supplemented with 100.0 mg·kg^(-1)Bm yeast cell wall, the ACP and ALP activities in serum and muscle of Penaeus vannamei were significantly boosted(t-test,P<0.05), while the ACP ,ALP and POD activities in hepatopancreas of Penaeus vannamei were strikingly significantly boosted (t-test,P<0.01).There were no significant differences among groups with different ratio of yeast cell wall (t-test,P>0.05). The study showed that when yeast cell wall were added in the feed at the ratio of 100.0 mg·kg^(-1)Bm,satisfying results can be achieved.

关 键 词：免疫多糖 酵母细胞壁 南美白对虾 免疫刺激剂 哈维氏弧菌 

分 类 号：S945.4[农业科学—水产养殖]