基因重组人骨形成蛋白-2胶原膜复合物防止大鼠矢状缝扩张后复发的作用  

Effects of rhBMP-2 Collagen Composite on Prevention of the Relapse of Rat Interparietal Suture after Rapid Expansion.

作　　者：周志迎[1] 赖仁发[1] 肖珲[2] 

机构地区：[1]暨南大学附属第一医院口腔科,广东广州510055 [2]广东省口腔医院正畸科

出　　处：《广东牙病防治》2004年第3期163-165,i001,共4页Journal of Dental Prevention and Treatment

基　　金：国家教委留学回国人员科研基金 (2 4 50 2 7);广东省医学科研基金 (A2 0 0 0 337)资助项目

摘　　要：目的　研究基因重组人骨形成蛋白 2 (rhBMP 2 )胶原膜复合物对大鼠顶骨矢状缝扩张后复发的影响。方法　 10周龄SD雄性大鼠 32只 ,随机分为空白对照组、单纯扩张组、扩张加胶原膜植入组、扩张加rhBMP 2胶原膜复合物植入组 ,每组 8只。大鼠左右顶骨钻孔 ,建立矢状缝扩张模型 ,第 2 1天时取出扩张装置 ,第 2 8天时处死动物 ;用游标卡尺测量相应时间点骨孔间的距离并计算扩张复发率。动物处死后完整取下顶骨 ,去除表面的软组织 ,采用组织学方法观察各组大鼠矢状缝处组织改建情况 ,用原子吸收分光光度计检测该处的钙含量。结果　施加扩张力后 ,矢状缝中新骨形成活跃 ,rhBMP 2胶原膜复合物植入组形成特有的骨质桥连接 ,钙含量显著高于其他组 (P <0 .0 1) ,扩张复发率则明显降低 (P <0 .0 1)。结论　rhBMP 2胶原膜复合物可以降低扩张力作用下大鼠顶骨矢状缝的扩张复发率 ,为临床上降低扩弓复发率提供新的思路。Objective To investigate the effects of rhBMP-2 collagen composite on bone regeneration during expansion of the interparietal suture in rats. Methods 32 10-week-old SD rats were divided into 4 groups with 8 rats each, including normal control, expansion control, atelo-type Ⅰ collagen and rhBMP-2 collagen composite group. The expansion sizes on 21th and 28th day were measured with vernier caliper and relapse was estimated. The bone regeneration in the interparietal suture was studied by histology while the calcium content was measured by atomic absorption spectrophotometer. Results The bone regeneration was more active in the suture after giving an expanding force than in the suture without any intervention. In rhBMP-2 collagen composite group bone bridge was formed and calcium content was much higher than in other three groups (P<0.01). Conclusion It was suggested that rhBMP-2 collagen composite could promote the bone regeneration in the expanded suture and it may be of therapeutic benefit in inhibiting relapse and shortenting the retention period through acceleration of bone regeneration in the midpalatal suture.

关 键 词：基因重组人骨形成蛋白-2胶原膜复合物 大鼠 矢状缝扩张 复发 预防 

分 类 号：R783[医药卫生—口腔医学]