Analysis of polymorphisms in the promoter region of interleukin-1 β by restriction fragment length polymorphism-PCR  

作　　者：文爱清 王军 冯凯 朱佩芳 蒋建新 

机构地区：[1]Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China

出　　处：《Chinese Journal of Traumatology》2004年第5期271-274,共4页中华创伤杂志（英文版）

基　　金：ThisworkwassupportedbytheKeyStateDevelopmentProgramforBasicResearchofChina (G1990 5 4 2 0 0 )andgrantsfrom86 3

摘　　要：Objective: To investigate the frequencies of -1470, (-511) and -31 single nucleotide polymorphisms (SNPs) in the promoter of IL-1β and its haplotype constitution in Chongqing population. Methods: One hundred and twelve healthy Chongqing people were enrolled in this study. Polymorphisms at -1470 (G to C), (-511) (T to C) and -31 (C to T) of IL-1β were genotyped with the method of restriction fragment length polymorphism (RFLP). Haplotype frequencies were analyzed by Arlequine software.Results: Frequencies of IL-1β -1470, -511 and -31 SNPs were (41.67)%, 50% and (45.33)%, respectively. Genotype frequencies of -1470 locus were (39.81)%, (37.04)% and (23.15)% for G/G, G/C and C/C respectively. As for T-511C SNP, genotype frequencies of (T/T), T/C and C/C were (29.91)%, (40.18)% and (29.91)%, respectively. Genotyping results of C/C, C/T, and T/T of -31 locus were (35.51)%, (38.32)% and (26.71)% respectively. Haplotype analysis found that there were mainly three haplotypes constituted by three SNPs, ie., G-T-C, C-T-C and G-C-T. Conclusions: Polymorphisms exist in the promoter of IL-1β in Chongqing population. Three SNPs locate in the same haplotype block.

关 键 词：INTERLEUKIN-1 Polymorphism  restriction fragment length Polymorphisms  single nucleotide GENOTYPE Haplotypes 

分 类 号：R346[医药卫生—基础医学]