肝癌SMMC-7721细胞中PKB对β-连环蛋白的调控  

Regulation of β-catenin by PKB in Human Hepatocellular Carcinoma Cell Line SMMC-7721

作　　者：范恺谊[1] 尹淼[1] 朱晓群[1] 徐莺莺[1] 申宗侯[1] 

机构地区：[1]复旦大学上海医学院生物化学与分子生物学系,上海200032

出　　处：《复旦学报（医学版）》2004年第5期445-448,共4页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金项目 ( 3 9970 3 3 8);上海市教委重点 ( 8990 80 6)资助项目

摘　　要：目的　探讨在肝癌SMMC - 772 1细胞中蛋白激酶B(PKB)对 β 连环蛋白 (β catenin)的调控作用。 方法用WesternBlot和RT PCR等研究在肝癌SMMC - 772 1细胞中激活或抑制PKB后 ,β catenin基因及蛋白水平的变化。结果　以H2 O2 活化PKB ,则SMMC - 772 1细胞胞质内游离的 β catenin水平明显升高 ,而与E cadherin结合的 β catenin水平没有变化 ,β catenin的mRNA表达也未见明显改变 ;用Wortmannin抑制PKB的活性 ,可使胞质内 β catenin的含量下降。 结论　肝癌SMMC - 772 1细胞中PKB可以调控胞质中游离的 β catenin ,这为深入研究 βPurpose: To study the regulation of β-catenin by activating and inactivating protein kianase B (PKB) in human hepatocellular carcinoma cell line SMMC-7721. Methods: The effects of H2O2 and Wortmannin on levels of PKB protein, phosphorylation of PKB and β-catenin protein were studied by Western blot. The levels of β-catenin mRNA treated with H 2O2 and Wortmannin were detected by RT-PCR. Results: In our research, when SMMC - 7721 cells were treated with H2O 2, which can activate PKB/Akt via PI3K pathway, the protein level of free β-catenin in cytoplasma was up-regulated, while there's no change in mRNA expression of β-catenin. Contrarily, when the cells were treated by PI3K special inhibitor, Wortmannin, β-catenin in cytoplasma was down-regulated. Conclusions: These results suggest that PKB can regulate free β-catenin in human hepatocellular carcinoma cell line SMMC-7721.

关 键 词：β-catenin SMMC-7721细胞 肝癌 Β-连环蛋白 调控 癌发生 mRNA表达 蛋白激酶B 

分 类 号：R735[医药卫生—肿瘤] R730[医药卫生—临床医学]