双氯芬酸钠对角膜上皮细胞抑制作用的实验研究  

An Investigation of the Effect and Mechanism of Diclofenac Sodium on Inhibiting of Corneal Epithelial Cells

作　　者：吴宁玲[1,2] 杜之渝 汤为学[3] 

机构地区：[1]重庆医学大学附属第二医院眼科中心,400010 [2]北京中国中医研究院眼科医院 [3]重庆医科大学病理生理教研室

出　　处：《中国眼耳鼻喉科杂志》2004年第5期282-284,i001,i002,共5页Chinese Journal of Ophthalmology and Otorhinolaryngology

基　　金：重庆市医学科技计划资助(00-3013)

摘　　要：目的探讨双氯芬酸钠对角膜上皮细胞体外增殖的抑制作用并初步探讨其机理。方法选用原代及传代兔角膜上皮细胞,实验组中加入含不同浓度双氯芬酸钠的培养液,对照组加入等量空白培养液,采用四甲基偶氮唑蓝(methyl thiazolyl thiazolium,MTT)比色法检测其对细胞增殖的抑制,光学显微镜和透射电子显微镜进行形态学观察。结果双氯芬酸钠浓度为27.27μg/ml～54.55 μg/ml 时,作用24、48、72 h 均明显抑制角膜上皮细胞的增殖(P<0.01),并具有剂量依赖性。结论双氯芬酸钠对角膜上皮细胞的增殖具有明显抑制作用,呈现剂量-效应关系。双氯芬酸钠可能是通过诱导凋亡而发挥其抗增殖作用。Purpose To discusss the effect of diclofenac sodium on inhibiting of rabbit corneal epithelial cells (RCECs)and explore its mechanism.Methods The primary culture and subculture of RCECs were exposed to different concentrations of diclofenac sodium in experimental group and to culture media in contrast group.The inhibtive characteristics of diclofenac sodium to the cells'proliferation were measured with methyl thiazolyl thiazolium(MTT)assay.The ultrastructure of cell were observed with light microscope and transmission electron microscope.Results After the treatment of 27.27 μg/ ml～54.55 μg/ml diclofenac sodium,RCECs showd the marked proliferation of inhibition in 24h,48h or 72h(P<0.01)and showed dosage dependence.Conclusion Diclofenac sodium has obvious inhibitive effect on RCECs and showed dosage-effect relation.Diclofenac sodium may exert its antiproliferative effect by inducing apoptusis.

关 键 词：双氯芬酸钠 角膜上皮细胞 抑制作用 抗增殖作用 实验研究 四甲基偶氮唑蓝 对照组 培养液 体外增殖 传代 

分 类 号：R772[医药卫生—眼科] R779.63[医药卫生—临床医学]