复方尼尔雌醇片抗大鼠骨质疏松的研究  

Compound nylestriol tablet to prevent bone loss in osteoporotic rats

作　　者：刘石平[1] 廖二元[1] 伍贤平[1] 罗湘杭[1] 李焕德[2] 程泽能[2] 戴如春[1] 孙瑞元 刘锡玖 

机构地区：[1]中南大学湘雅二医院代谢内分泌研究所 [2]中南大学湘雅二医院药剂科,长沙410011 [3]安徽省药师临床评价中心,芜湖241001

出　　处：《中南大学学报（医学版）》2004年第4期405-409,共5页Journal of Central South University ：Medical Science

基　　金：国家"九五"攻关项目 (96 90 6 0 5 0 3) ;国家"十五"攻关项目 (2 0 0 1BA70 2B0 3) ;湖南省社会发展科研计划 (99SSY1 0 0 9 1 0 )

摘　　要：目的 :观察复方尼尔雌醇片 (CNT)对大鼠骨质疏松的作用 ,并与其组分进行比较。方法 :1 4 4只 7月龄雌性SD大鼠随机分为 1 2组 ,每组 1 2只 ;另外 1 2 0只 4月龄雌性SD大鼠随机分为 1 2组 ,每组 1 0只。采用随机区组设计分别比较CNT(每日 0 .0 39,0 .1 1 7,0 .39mg/kg体重 )及其组分 (尼尔雌醇 0 .30 ,0 .0 9,0 .0 3mg/kg体重或左炔诺孕酮 0 .0 9,0 .0 2 7,0 .0 0 9mg/kg体重 ) 3种剂量水平对维甲酸和去卵巢所致骨质疏松SD大鼠的作用。测定大鼠血钙、磷、甘油三酯 (TG)、总胆固醇 (TC)、碱性磷酸酶 (ALP)、高密度脂蛋白胆固醇 (HDL C)、全身骨密度 (BMD)、离体左股骨BMD及股骨灰化后的钙、磷含量。结果 :①用每日 70mg/kg体重的维甲酸灌胃或去卵巢均可成功制备大鼠骨质疏松模型。②CNT及其组分有抗两种大鼠模型的骨丢失作用 ;③CNT的作用强于同剂量的尼尔雌醇和左炔诺孕酮 ,且呈剂量依赖性。④在多数观察指标中 ,CNT大、中剂量水平的作用类似 ,故以中等剂量水平 (0 .1 1 7mg/kg)为优选。结论 :CNT可阻止维甲酸和去卵巢所致SD大鼠的骨丢失 。Objective To investigate the effects of compound nylestriol tablet (CNT),containing nylestriol and levonorgestrel with a ratio of 1:0.3) and its components on the osteoporotic rat model induced by retinoic acid (RA) and ovariectomy (OVX).Methods We randomly divided 144 female SD rats (aged 7 month old) into 12 groups (12 in each). In addition,120 female SD rats aged 4 month were randomly divided into 10 groups (10 in each). Three dosage levels of CNT (0.039, 0.117 and 0.39 mg/kg body weight, daily), nylestriol (NYL, 0.30, 0.09 and 0.03 mg/kg body weight, daily) and levonorgestrel (LEV, 0.09, 0.027 and 0.009 mg/kg body weight, daily) were designed to prevent the bone loss of the osteoporotic rat model induced by RA and OVX respectively. Serum total calcium (Ca),phosphate (Pi), ALP, triglyceride (TG), total cholesterol (TC), HDL C, total body BMD, isolated left femoral BMD and femoral Ca,and Pi after ashing were determined.Results Osteoporotic models could be induced by RA (70 mg/kg body weightdaily given intragastrically for 14 days) or by ovariectomy;CNT and its components had the preventive effects on bone loss in both models;The preventive effect of CNT was dose dependent and stronger than its components;Many observed markers showed us the levels of high and moderate CNT given to be the strongest,corresponding to an optimal choice of the moderate dose CNT about one tablet per week in adult women. Conclusions CNT therapy can prevent the bone loss of RA induced and OVX induced osteoporotic rats,and its therapeutic and preventive effect is better than its components used alone.

关 键 词：尼尔雌醇 SD大鼠 骨质疏松 去卵巢 维甲酸 复方 骨丢失 雌性 水平 碱性磷酸酶 

分 类 号：R58[医药卫生—内分泌]