人重组骨形态发生蛋白-2修复全层关节软骨缺损的实验研究  

Experimental study on repair of full-thickness articular cartilage defects with recombinant human bone morphogenetic protein-2

作　　者：陈旸[1] 韩培[1] 邵俊杰[1] 蒋垚[1] 

机构地区：[1]上海交通大学附属第六人民医院骨科,上海200233

出　　处：《中国矫形外科杂志》2004年第19期1483-1485,共3页Orthopedic Journal of China

基　　金：上海市科学技术委员会资助项目 (NO 0 1 4 1 1 90 71 )

摘　　要：目的 :研究人重组骨形态发生蛋白 2 (rhBMP 2 )对全层关节软骨缺损的修复 ,观察修复效果。方法 :家犬 8只 (16膝 ) ,每个膝内外髁均做全层软骨缺损 ,内外髁共 3 2个缺损。随机分为 4组 ,每组 2只。每只犬一侧关节行胶原海绵吸附rh BMP填充内外髁缺损 ,另一侧以单纯胶原海绵填充作对照 ,不处理组为空白对照。术后 2、4、 8、 12周取材作大体、光镜、透射电镜观察。结果 :rh BMP组为类软骨细胞修复 ,而单纯胶原海绵组和空白组均为纤维性修复。结论 :rhBMP 2有效地促进关节软骨缺损的修复 。Objective:To study rhBMP 2 on repair of full thickness articular cartilage defects,and to observe the effect Method:Full thickness articular cartilage defects were created in each femoral condyle of 8 dogs The 8 dogs were randomly divided into 4 groups,one knee of every dog was filled with a collagen sponge impregnated 5 micrograms of rhBMP 2 The defects of other knees were filled with a collagen sponge only or nothing Obtained samples at 2,4,8,12 weeks after operation and observed by gross,optical and electronic microscopic and immunohistochemical analysis Result:The group of rhBMP 2 had a significantly better histological appearance cartilage repair than the group of a collagen sponge only and the group of empty Conclusion:rhBMP 2 can effectively repair full thickness articular cartilage defect

关 键 词：关节软骨 软骨缺损 RHBMP-2 

分 类 号：R684[医药卫生—骨科学]