心肌细胞中的钙火花(英文)  

Calcium Sparks in Heart Muscle Cells

作　　者：程和平[1] 沈建新[2] 

机构地区：[1]北京大学生命科学学院膜与膜生物工程国家实验室,北京100871 [2]汕头大学医学院生理学教研室

出　　处：《中山大学学报（医学科学版）》2004年第5期387-389,394,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家重大基础研究发展资助项目(G2000057002);广东省卫生厅"五个一"工程重点基金资助项目(413626)

摘　　要：细胞离子钙是最普遍、最重要的第二信使之一,参与包括肌肉收缩、突触传递、激素分泌、基因转录以及细胞分裂、受精、代谢和凝血等多种生理过程。对心肌细胞来说,离子钙将电活动与收缩联系起来,即介导细胞的兴奋-收缩耦联。1993年我们首次在激光共聚焦显微镜下发现心肌细胞胞内钙释放的基本单位———钙火花。历经十几年的研究,人们对钙火花和胞内钙信号的本质及兴奋-收缩耦联的微观机理的认识日趋深刻。特别是,新发展起来的非紧密封接膜片钳和激光共聚焦显微镜成像合成技术可以激活单个L型钙通道,通过钙致钙释放机制,诱发钙火花。此文简要介绍心肌细胞中自发和诱发钙火花的研究及其意义,并对有关研究领域的前景作一展望。As a ubiquitous intracellular messe nger,Ca 2+ plays important roles in vital physiological processes,such as mu scle contraction,synaptic transmi ssion,hormone secretion,metabolism,gene transcription,fe rtilization,cell survival and cell death.It has been increasingly appreciated that spatial and temporal patterning endows Ca2+ signal with efficiency,specificity,and unparalleled versatility,such that the same ions fulfill different and even opposing physiological functions in a given cell.Thus,dire ct visualization of Ca 2+ microdomain(0.1-10μm)holds the promise to dissect out the space-tim e architecture of Ca 2+ dynamics,and thereby to elucidate C a 2+ signaling mechanisms in different physiological contexts.Since our first recording of Ca 2+ sparks in cardiac myocytes in 1993,imaging mi crodomain Ca2+ in muscles has offered unique insigh ts into molecular mechanisms of excitation-contraction(EC)coupling,and revolutionized our understanding of Ca2+ handling and signal transduction(for reviews,see Reference 7).In this mini-review,we intend to present a brief introducti on on Ca 2+ sparks in cardiac myocytes and other cell types.

关 键 词：心肌细胞 钙火花 激光共聚焦显微镜 离子钙 诱发 发钙 兴奋 钙释放 第二信使 钙信号 

分 类 号：R363[医药卫生—病理学] Q257[医药卫生—基础医学]