T细胞膜脂肪微区域与白细胞介素-2α受体  

Interleukin-2α receptor in membrane lipid rafts

作　　者：李秋荣[1] 马健[1] 汪灏[1] 黎介寿[1] 

机构地区：[1]南京军区南京总医院解放军普通外科研究所,江苏南京210002

出　　处：《肠外与肠内营养》2004年第5期262-264,共3页Parenteral & Enteral Nutrition

基　　金：国家重点基础研究发展规划 ( 2 0 0 3CB5 15 5 0 2 );南京军区南京总医院科研基金 ( 2 0 0 40 3 4)项目

摘　　要：目的 :确定T细胞膜脂肪微区域 (lipidrafts )是IL 2Rα的功能性区域。　方法 :应用流式细胞仪检测T细胞表面分子CD2 5 (IL 2Rα)的表达 ,分离T细胞膜脂肪微区域 ,应用蛋白质印迹法检测分析IL 2Rα所在的T细胞膜脂肪微区域分离组分。　结果 :在IL 2刺激下 ,T细胞膜表面分子CD2 5 (IL 2Rα)阳性表达率为 37.0 8% ,蛋白质印迹法检测确定IL 2Rα存在于脂肪微区域组分。　结论 :T细胞膜脂肪微区域是IL 2Rα的功能性区域。Objective: To determine lipid rafts on the membrane of T cells is the functional microdomains for IL-2α receptor. Methods: Flow cytometric analysis was performed for expression of CD25(IL-2Rα) on the surface of T cells. Lipid rafts were isolated by discontinuous sucrose density gradient ultracentrifugation. The localization of IL-2Rα in fractions isolated from sucrose density gradients was determined by immunoblotting and detected by chemiluminescence. Results: cells were stimulated with IL-2 and expression of CD25(IL-2Rα) on the surface of T cells was 37.08%. Immunoblot analysis of fractions from sucrose gradients revealed that a large proportion of IL-2Rα was localized in lipid rafts. Conclusions: lipid rafts is the functional microdomains for IL-2α receptors.

关 键 词：T细胞 IL-2RΑ 脂肪 Α受体 蛋白质印迹法 CD25 白细胞介素-2 

分 类 号：Q26[生物学—细胞生物学]