硬脂酸聚乙二醇阿霉素纳米粒的制备及其对人肝癌细胞的杀伤作用  

Stearic acid-polyethylene glycol-doxorubincin nanoparticles′ preparation and it′s effect on killing hepatocarcinoma cells in human being

作　　者：李志花[1] 陈汝福[2] 王捷[2] 陈积圣[2] 

机构地区：[1]中山大学附属第二医院肿瘤科,广州510120 [2]中山大学附属第二医院肝胆外科,广州510120

出　　处：《岭南现代临床外科》2004年第3期171-174,共4页Lingnan Modern Clinics in Surgery

基　　金：广东省医学科学研究基金(A2002201);广东省自然科学基金(2003A031700)

摘　　要：目的合成硬脂酸聚乙二醇阿霉素纳米微粒(DOX鄄SLNs鄄PEG)并检测其相关参数,观察其对人肝癌细胞杀伤作用。方法以“乳化蒸发鄄低温固化”法合成DOX鄄SLNs鄄PEG纳米粒。透射电镜计算粒径,分光光度法计算载药率,噻唑蓝法(MTT法)观察对肝癌细胞的体外杀伤效应及观察体内抑瘤效应。结果DOX鄄SLNs鄄PEG纳米粒平均粒径120±4.84mm,包封率68.6%,体外释放实验提示,7d可释放所载60%左右的药物。体内抑瘤实验表明:控释制剂间隔给药疗效已优于未包载药物每日给药的疗效,量效关系明显。结论DOX鄄SLNs鄄PEG纳米粒可有效抑制肝癌细胞的生长。Objectives To prepare DOX-SLNs-PEG nanoparticles and detect their correlative parameters and their effect on killing hepatocarcinoma cells in human being. Methods The DOX-SLNs-PEG nanoparticles were prepared by the method of 'emulsion evaporation-solidification at low temperature'.The diameter of nanoparticles was determined by transmission electron microphotography,the drug-carrying amount of DOX-SLNs-PEG nanoparticles was calculated by spectrophotometer. Killing effect in vitro and inhibiting effect in vivo of hepatocareinoma cells were observed by MTT assay.Results The average diameter value of DOX-SLNs-PEG nanoparticles was 120±4.84mm.Envelope rate is 68.6%.The releasing experimentation in vitro indicated that DOX-SLNs-PET nanoparticles could release nearly 60% drugs of their carriages in 7 days.Exhebiting experimentation in vivo revealed the efficacy of control-releasing agent by interval medication which is much superior to the unenveloped drug by every day medication.The relationship between dosage and efficacy were showed obviously.Conclusion DOX-SLNs-PEG nanoparticles can inhibit effectively the growth of hepatocarcinoma cell.

关 键 词：SLN 纳米粒 人肝癌细胞 杀伤作用 阿霉素 载药 体内 观察 药物 PEG 

分 类 号：R735[医药卫生—肿瘤] R944[医药卫生—临床医学]