Gene expression profile in liver of hB1F transgenic mice  

作　　者：Shui-LiangWang HuaYang You-HuaXie YuanWang Jian-ZhongLi LongWang Zhu-GangWang Ji-LiangFu 

机构地区：[1]DepartmentofMedicalGenetics,SecondMilitaryMedicalUniversity,Shanghai200433,China [2]StateKeyLaboratoryforMolecularBiology,InstituteofBiochemistryandCellBiology,ShanghaiInstitutesforBiologicalSciences,ChineseAcademyofSciences,Shanghai200031,China [3]ShanghaiNanfangResearchCenterforModelOrganisms,Shanghai201203,China

出　　处：《World Journal of Gastroenterology》2004年第20期3006-3010,共5页世界胃肠病学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China,No.39830360; the National "863" High Technology Research and Development Program of China,No.2001AA221261; the Qi Ming Xing Program from Shanghai Science and Technology Committee,No.01QA 140

摘　　要：AIM: To analyze the tissue morphologic phenotype and liver gene expression profile of hBIF transgenic mice. METHODS: Transgene expression was analyzed with RT-PCR and Western blotting. For one of the transgenic mouse lines, tissue expression pattern of the transgene was also examined with immunochemical methods. Pathological analysis was used to examine the tissue morphologic phenotype of established transgenic mice. The liver gene expression profile of transgenic mice was analyzed with microchip, and some of bhe differentially expressed genes were verified with RT-PCR. RESULTS: The expressions of hBIF were shown in livers from 6 of 7 transgenic mouse lines. The overexpression of hB1F transgene did not cause pathological changes. Expressions of three genes were up-regulated, while down-regulation was observed for 25 genes. CONCLUSION: The overexpression of hBIF transgene may cause changes of gene expression profiles in the liver of transgenic mice.AIM:To analyze the tissue morphologic phenotype and liver gene expression profile of hB1F transgenic mice. METHODS:Transgene expression was analyzed with RT- PCR and Western blotting.For one of the transgenic mouse lines,tissue expression pattern of the transgene was also examined with immunochemical methods.Pathological analysis was used to examine the tissue morphologic phenotype of established transgenic mice.The liver gene expression profile of transgenic mice was analyzed with microchip,and some of the differentially expressed genes were verified with RT-PCR. RESULTS:The expressions of hB1F were shown in livers from 6 of 7 transgenic mouse lines.The overexpression of hB1F transgene did not cause pathological changes.Expressions of three genes were up-regulated,while down-regulation was observed for 25 genes. CONCLUSION:The overexpression of hB1F transgene may cause changes of gene expression profiles in the liver of transgenic mice.

关 键 词：基因表达 肝脏 HB1F 遗传转移 老鼠 病理学 

分 类 号：R575[医药卫生—消化系统]