Anti-tumor effect of pEgr-IFNγgene-radiotherapy in B16 melanoma-bearing mice  

作　　者：Cong-MeiWu Xiu-YiLi Tian-HuaHuang 

机构地区：[1]ResearchCenterofReproductiveMedicine,ShantouUniversityMedicalCollege(SUMC),Shantou515041,GuangdongProvince,China [2]TheMinistryofPublicHealthRadiobiologyResearchUnitofJilinUniversity,Changchun130021,JilinProvince,China

出　　处：《World Journal of Gastroenterology》2004年第20期3011-3015,共5页世界胃肠病学杂志（英文版）

基　　金：Supported by the National Natural Science Fundation of China,No.39970229

摘　　要：AIM: To construct a pEgr-IFNγ Plasmid and to investigate its expression properties of interferon-γ (INF-γ) induced by irradiation and the effect of gene-radiotherapy on the growth of melanoma. METHODS: A recombined plasmid, pEgr-IFNγ, was constructed and transfected into B16 cell line with lipofectamine. The expression properties of pEgr-IFNγ were investigated by ELISA. Then, a B16 melanoma-bearing model was established in mice, and the plasmid wasinjected into the tumor tissue. The tumor received 20 Gy X-ray irradiation 36 h after injection, and IFN-γ expression was detected from the tumor tissue. A tumor growth curve at different time points was determined. RESULTS: The eukaryotic expression vector, pEgr-IFNγ, was successfully constructed and transfected into B16 cells. IFN-γ expression was significantly increased in transfected cells after X-ray irradiation in comparison with 0 Gy group (77.73-94.60 pg/mL, P<0.05-0.001), and was significantly higher at 4 h and 6 h than that of control group after 2 Gy X-ray irradiation (78.90-90.00 pg/mL, P<0.01-0.001).When the transfected cells were given 2 Gy irradiation 5 times at an interval of 24 h, IFN-y expression decreased in a time-dependent manner. From d 3 to d 15 after IFNγ generadiotherapy, the tumor growth was significantly slower than that after irradiation or gene therapy alone. CONCLUSION: The anti-tumor effect of pEgr-IFNγ generadiotherapy is better than that of genebherapy or radiotherapy alone for melanoma. These results may establish an important experimental basis for gene-radiotherapy of cancer.AIM:To construct a pEgr-IFNy plasmid and to investigate its expression properties of interferon-γ(INF-γ)induced by irradiation and the effect of gene-radiotherapy on the growth of melanoma. METHODS:A recombined plasmid,pEgr-IFNγ,was constructed and transfected into B16 cell line with lipofectamine.The expression properties of pEgr-IFNγ were investigated by ELISA.Then,a B16 melanoma-bearing model was established in mice,and the plasmid was injected into the tumor tissue.The tumor received 20 Gy X-ray irradiation 36 h after injection,and IFN-γ expression was detected from the tumor tissue.A tumor growth curve at different time points was determined. RESULTS:The eukaryotic expression vector,pEgr-IFNγ, was successfully constructed and transfected into B16 cells. IFN-γ,expression was significantly increased in transfected cells after X-ray irradiation in comparison with 0 Gy group (77.73-94.60 pg/mL,P<0.05-0.001),and was significantly higher at 4 h and 6 h than that of control group after 2 Gy X-ray irradiation(78.90-90.00 pg/mL,P<0.01-0.001). When the transfected cells were given 2 Gy irradiation 5 times at an interval of 24 h,IFN-γ expression decreased in a time-dependent manner.From d 3 to d 15 after IFNγ gene- radiotherapy,the tumor growth was significantly slower than that after irradiation or gene therapy alone. CONCLUSION:The anti-tumor effect of pEgr-IFNγ gene- radiotherapy is better than that of genetherapy or radiotherapy alone for melanoma.These results may establish an important experimental basis for gene-radiotherapy of cancer.

关 键 词：pEgr-IFNγ 基因 放射线疗法 B16 黑素瘤 老鼠 X-射线 

分 类 号：R739.5[医药卫生—肿瘤]