Effect of resveratrol and in combination with 5-FU on murine liver cancer  

作　　者：Sheng-LiWu Zhong-Jiesun LiangYu Ke-WeiMeng Xing-LeiQin Cheng-EnPan Sheng-LiWu 

机构地区：[1]DepartmentofHepatobiliarySurgery,FirstHospitalofXi'anJiaotongUniversity,Xi'an710061,ShaanxiProvince,China [2]DepartmentofHepatobiliarySurgery,People'sHospitalofShaanxiProvince,Xi'an710061,ShaanxiProvince,China [3]DepartmentofHepatobiliarySurgery,FirstHospitalofXi'anJiaotongUniversity,Xi'an710061,ShaanxiProvince,China

出　　处：《World Journal of Gastroenterology》2004年第20期3048-3052,共5页世界胃肠病学杂志（英文版）

基　　金：Supported by Traditional Chinese Medicine Bureau Foundation of Shaanxi Province,No.2001-035

摘　　要：AIM: To study the anti-tumor effect of resveratrol and in combination with 5-FU on murine liver cancer. METHODS: Transplantable murine hepatoma22 model was used to evaluate the anti-tumor activity of resveratrol (RES) alone or in combination with 5-FU in vivo. H22 cell cycles were analyzed with flow cytometry. RESULTS: Resveratrol could inhibit the growth of murine hepatoma22, after the mice bearing H22 tumor were treated with 10 mg/kg or 15 mg/kg resveratrol for ten days, and the inhibition rates were 36.3% (n = 10) and 49.3% (n = 9), respectively, which increased obviously compared with that in control group (85+22 vs 68+17, P<0.01). RES could induce the S phase arrest of H22 cells, and increase the persentage of cells in S phase from 59.1% (n = 9) to 73.5% (n = 9) in a dose-dependent manner (P<0.05). The enhanced inhibition of tumor growth by 5-FU was also observed in hepatoma22 bearing mice when 5-FU was administered in combination with 10 mg/kg resveratrol. The inhibition ratesfor 20 mg/kg or 10 mg/kg 5-FU in combination with 10 mg/kg resveratrol were 77.4% and 72.4%, respectively, compared with the group of 20 mg/kg or 10 mg/kg 5-FU alone, in which the inhibition rates were 53.4% and 43.8%, respectively (n = 8). There was a statistical significance between the combination group and 5-FU group. CONCLUSION: RES could induce the S phase arrest of H22 cells and enhance the anti-tumor effect of 5-FU on murine hepatoma22 and antagonize its toxicity markedly. These results suggest that resveratrol, as a biochemical modulator to enhance the therapeutic effects of 5-FU, may be potentially useful in cancer chemotherapy.AIM:To study the anti-tumor effect of resveratrol and in combination with 5-FU on murine liver cancer. METHODS:Transplantable murine hepatoma_(22) model was used to evaluate the anti-tumor activity of resveratrol(RES) alone or in combination with 5-FU in vivo.H_(22) cell cycles were analyzed with flow cytometry. RESULTS:Resveratrol could inhibit the growth of murine hepatoma_(22),after the mice bearing H_(22) tumor were treated with 10 mg/kg or 15 mg/kg resveratrol for ten days,and the inhibition rates were 36.3%(n=10)and 49.3%(n=9), respectively,which increased obviously compared with that in control group(85±22 vs 68±17,P<0.01).RES could induce the S phase arrest of H_(22) cells,and increase the persentage of cells in S phase from 59.1%(n=9)to 73.5% (n=9)in a dose-dependent manner(P<0.05).The enhanced inhibition of tumor growth by 5-FU was also observed in hepatoma_(22) bearing mice when 5-FU was administered in combination with 10 mg/kg resveratrol.The inhibition rates for 20 mg/kg or 10 mg/kg 5-FU in combination with 10 mg/kg resveratrol were 77.4% and 72.4%,respectively,compared with the group of 20 mg/kg or 10 mg/kg 5-FU alone,in which the inhibition rates were 53.4% and 43.8%,respectively (n=8).There was a statistical significance between the combination group and 5-FU group. CONCLUSION:RES could induce the S phase arrest of H_(22) cells and enhance the anti-tumor effect of 5-FU on murine hepatoma_(22) and antagonize its toxicity markedly.These results suggest that resveratrol,as a biochemical modulator to enhance the therapeutic effects of 5-FU,may be potentially useful in cancer chemotherapy.

关 键 词：白藜芦醇 化合物 5-FU 鼠 肝癌 肿瘤 血细胞记数 

分 类 号：R735.7[医药卫生—肿瘤]