肺癌患者MAGE-A3抗原表达及HLA-I类基因分布的初步研究  

Detection of MAGE-A3Antigen and HLA-class I Gene Distribution in Lung Cancer

作　　者：庄东刚[1] 吴逸明[1] 巴月[1] 

机构地区：[1]郑州大学医学院公共卫生学院,河南郑州450052

出　　处：《河南预防医学杂志》2004年第4期193-196,199,共5页Henan Journal of Preventive Medicine

基　　金：河南省自然科学基金资助项目 (0 1110 2 14 0 0 ) ;河南省科技攻关计划资助项目 (0 12 4 170 2 32 )

摘　　要：目的　检测MAGE -A3抗原在肺癌组织中的表达情况以及HLA -I类基因在肺癌患者中的分布水平 ,以预测能够应用以MAGE -A3抗原蛋白为基础的肿瘤疫苗进行肿瘤免疫治疗的肺癌患者的比例。方法　用SDS -PAGE和Westernblot方法检测 6 3例肺癌及其癌旁组织MAGE -A3抗原的表达情况 ;用PCR -SSP方法检测 70例肺癌及癌旁组织HLA -A1、A2、A2 4的分布频率。结果　 6 3例肺癌患者中有 31例为MAGE -A3抗原表达阳性 ,其中 5例癌旁组织MAGE -A3抗原也为阳性。 70例肺癌患者中HLA -A1、A2、A2 4三个等位基因的分布频率分别为 4 .2 8%、5 4 .2 8%、5 0 .0 % ;70例癌旁组织中HLA -A1、A2和A2 4的阳性率分别为 4 .2 8%、6 0 .0 %和 5 2 .86 %。三等位基因中任一基因在肺癌组织出现的频率为 80 %。结论　肺癌患者中大约有 39%的个体能应用基于MAGE -A3抗原蛋白的肿瘤疫苗进行有效的肿瘤免疫治疗。Object:TO investigate the expression Of MAGE-A3 antigen and thedistribution Of HLA-class l gene in lung cancer tissues,and to predict the ratio Of lungcancer patients who can be treated with immunotherapy based on MAGE-A3 antigen.Methods:SDS-PAGE and Western blot analysis were used to detect the expression of MAGE-A3 antigen in 63 lung cancer patients(include tumor tissues and their adjacenttissues).PCR-SSP technique was used to detect the distribution of HLA-A1,A2 and A24 gene in 70 lung cancer patients and their tumor adjacent.Results Of 63 lung cancer patients,31 tumor tissues(49.21%)have MAGE-A3 antigen expression,and 5 tumor adjacent tissues also had MAGE-A3 antigen expression.Of 70 lung cancerpatients the distribution Of HLA-A1,A2 and A24 are 4.28%,54.28%,and 50.0%respectively.Of 70 tumor adjacents the distribution Of HLA-A1,A2,A24 are 4.28%,60.0%,and 52.86%respectively.The total positive rate Of HLA-A1,A2,A24 is 80%in lung cancer tissues.Conclusion:There are.about39%lungcancerpatients(80.0%×49.21%)who can be treated with immunotherapy based on MAGE-A3 antigen.

关 键 词：肺肿瘤 MAGE—A3 HLA—I 免疫治疗 

分 类 号：R734.2[医药卫生—肿瘤]