老年期痴呆患者血清Tau蛋白免疫印迹分析  

ANALYSIS OF TAU PROTEIN IN SERUM IN SENILE DEMENTIA PATIENTS USING IMMUNO - SLOT BLOT

作　　者：葛炜[1] 程吟梅[1] 张曙云[1] 叶善龙 陈俊杰[3] 何晓嫱[3] 马英红[3] 

机构地区：[1]解放军杭州疗养院,310007 [2]上海市长宁区老年痴呆专科医院 [3]华西医科大学重组DNA研究室

出　　处：《老年医学与保健》1999年第2期67-69,共3页Geriatrics & Health Care

摘　　要：目的 探讨血清Tau蛋白水平对老年期痴呆患者临床诊断的意义.方法 应用单克隆抗Tau蛋白抗体(Anti-Tau l)和免疫狭缝印迹(ISB)技术对112例老年期痴呆患者[Alzheimer病(AD)75例、多发性梗塞性痴呆(MID)37例]和24名年龄配对的老年健康人的血清测定Tau蛋白水平,用分子分析仪并联计算机(IBM)扫描进行分析,血清Tau蛋白的相对浓度以校准强度(CI)表示.结果 本研究显示老年期痴呆患者组的血清Tau蛋白含量(CI值:总计=0.114±0.03,AD=0.110 ±0.03,MID=0.123 ±0.04)与对照组(CI值为0.116±0.04)的差异无显著性(P值均>0.05),也不受有或无apo E_4等位基因(∈_4)的影响.结论 在临床上诊断老年期痴呆时,有必要采用相同技术对脑脊液(CSF)中的和血清中的Tau蛋白进行同步分析比较.ve To understand the significance of serum Tau protein level to aid in the clinical di-agnosis of patients with senile dementia. Methods Serum samples were collected from patients with Alzheimer' s disease (AD,n = 75) and multi - infarct dementia (MID,n = 37) and aged matched healthy subjects (n = 24) . The serum Tau protein was analyzed by using immuno - slot blot (ISB) with anti - Tau monoclonal antibodies and then scanned by using Molecular Analyzer associated with IBM system. The relative concentration of Tau protein was rep-resented as calibration intensity ( CI) . Results The results show that the mean content of serum Tau protein in se-nile dementia group ( CI: Total = 0.114±0?.03, AD = 0.110±0.03 and MID = 0.123±0.04) has not statistically different as compared with control group (CI = 0.116±0.04) and is not influenced by patients with or without apolipoprotein E4 genotype. Conclusions It is necessary for clinical to detect and comparative study on Tau pro-tein in CSF and serum in senile dementia patients (AD and MID) with the same (ISB) method.

关 键 词：TAU蛋白 老年期痴呆 血清 患者 免疫印迹分析 诊断 多发性梗塞性痴呆 

分 类 号：R749.16[医药卫生—神经病学与精神病学] R743[医药卫生—临床医学]