宫颈癌放疗前后肿瘤细胞凋亡及其相关基因的变化  

The Change of Cell Apoptosis and the Expression of Genes before and after Radiotherapy in Cervical Carcinoma

作　　者：董广璐[1] 张淑云[2] 邢丽娜[1] 郑淑香[1] 

机构地区：[1]哈尔滨医科大学附属第二医院放射治疗科,哈尔滨市150086 [2]哈尔滨医科大学附属第二医院科研实验中心

出　　处：《中国肿瘤临床》2004年第19期1098-1100,共3页Chinese Journal of Clinical Oncology

基　　金：黑龙江省自然科学基金资助(编号:0-9961)

摘　　要：目的:探讨宫颈癌组织放疗前后肿瘤细胞凋亡及凋亡调节基因p53、bcl-2和bax蛋白表达的变化及意义。方法:选择未经治疗的宫颈癌患者20例为试验对象,采集放疗前和放疗10Gy后宫颈癌组织标本,用原位DNA切口末端标记(TUNEL)法检测凋亡细胞;单克隆抗体免疫组化SABC法检测细胞凋亡相关基因p53、bcl-2和bax的蛋白表达水平。结果:1)在宫颈癌放疗前后,肿瘤细胞凋亡阳性率和平均凋亡指数分别为25.00％和0.11％、75.00％和3.40％,放疗前后有显著性差异(P<0.01);2)放疗前肿瘤细胞凋亡相关基因p53、bax和bcl-2蛋白表达阳性率分别为55%、10％和20％,而放疗后分别为25％、60%和25％,放疗后p53蛋白表达减少,bax蛋白明显增加,bcl-2蛋白无明显变化。结论:放射治疗诱导宫颈癌肿瘤细胞凋亡,可能与凋亡调节基因bax基因表达的诱导密切相关。Objective: To investigate the change of cell apoptosis and the expression of p53、bax and bcl-2 before and after radiotherapy in cervical carcinoma. Methods: Twenty patients with cervical carcinoma who received only radiotherapy were registered. Specimens of formalin-fixed, paraffin-embedded tissues were obtained from the same site in cervical tumors before and after administration of a irradiation dose of 10Gy. Cell apoptosis and the expression of p53 、 bax and bcl-2 proteins were assessed by using the method of in situ nick end labeling combined with hematoxylin and eosin staining and of immunohistochemistry, respectively. Results: 1) The apoptosis index was significantly increased from 0.11% before radiotherapy to 3.40% after 10Gy. 2) The expression of p53 and bax proteins showed significantly correlative variation after 10Gy, and no significant change was found in the expression rate of bcl-2 protein before and after radiotherapy in cervical carcinoma. Conclusion: Bax proteins were associated with apoptosis induced by radiotherapy.

关 键 词：宫颈癌放射治疗 细胞凋亡p53 BCL-2 BAX 

分 类 号：R737.33[医药卫生—肿瘤]