三种药物对人乳腺癌细胞体外杀伤作用比较  

Comparison of In Vitro Anti-cancer Activity of Pirarubicin,Adriamycin, and Epirubicin on Human Breast Cancer Cells

作　　者：田海梅[1] 傅军[1] 王燕[2] 石晓燕[1] 曹冬燕[1] 徐兵河[2] 张保宁[3] 张伟[1] 

机构地区：[1]中国医学科学院肿瘤研究所肿瘤生物学检测中心,北京市100021 [2]中国医学科学院肿瘤医院内科 [3]中国医学科学院肿瘤医院乳腺癌治疗中心

出　　处：《中国肿瘤临床》2004年第19期1113-1116,共4页Chinese Journal of Clinical Oncology

摘　　要：目的:比较吡柔比星(THP)和阿霉素(ADM)、表阿霉素(EPI)对人乳腺癌细胞的体外抑制及凋亡诱导作用,以及药物杀伤的时效关系。方法:采用ATP生物荧光肿瘤体外药敏检测技术(ATP-TCA),比较吡柔比星和阿霉素、表阿霉素对人乳腺癌细胞系MCF-7、T-47D和Bcap-37的生长抑制作用,并绘制细胞存活率-时间曲线;用Annexin V凋亡检测技术检测药物诱导细胞凋亡的情况。结果:3种药物对乳腺癌细胞系MCF-7、T-47D和Bcap-37的生长抑制作用呈现明显的剂量依赖性;吡柔比星对其生长抑制作用明显高于阿霉素和表阿霉素,其IC50仅相当于阿霉素和表阿霉素的1/3-1/6,与后两者的差异均具有显著意义(P<0.01);阿霉素和表阿霉素的抑制作用相当,两者IC50无显著性差异(P>0.05)。三种药物对乳腺癌细胞的抑制作用均呈时间依赖关系,低浓度的吡柔比星在短时间内(12h)即具有生长抑制作用,而阿霉素和表阿霉素的起效时间较为迟缓(24-36h),72h后肿瘤细胞的存活率较吡柔比星高10%-20％。3种乳腺癌细胞分别经低浓度的吡柔比星、阿霉素和表阿霉素处理后均可诱导凋亡,其中吡柔比星组的细胞凋亡比例较其它两组为高。结论:吡柔比星对乳腺癌细胞具有较好的生长抑制和凋亡诱导作用,其起效时间明显早于阿霉素和表阿霉素。该药良好的抑瘤效果和迅速的起效时间对?Objective: To compare the anti-cancer activity of pirarubicin, adriamycin, and epirubicin, on three breast cancer cell lines, MCF-7, T-47D, and Bcap-37, and to study their time-effect relationship. Methods: ATP based tumor chemosensitivity assay (ATP-TCA) was used to establish the in vitro dose-effect and time-effect curves. The ability of these three drugs to induce apoptosis of the three cell lines was determined by Annexin V apoptosis assay method. Results: The inhibition of the three drugs on the growth of MCF-7, T-47D, and Bcap-37 was dose-dependent. THP exhibited a stronger growth inhibition than adriamycin and epirubicin on three breast cancer cell lines, respectively. 50% inhibiting concentration (IC50) of THP for three cell lines was significantly lower than adriamycin and epirubicin (P<0.01), while the latter two drugs showed no statistical difference (P>0.05). The inhibitory effects of three drugs on breast cancer cells were time dependent. THP at low concentration exhibited growth inhibition on breast cancer cells rapidly (12-24h); while retarded effect was observed in adriamycin and epirubicin treated groups with 10-20% higher tumor survival rate than THP group. Increased apoptotic ratio of cancer cells was noted in all drug-treated groups, while THP treated group exhibited higher apoptotic ratio than that of the other two drug-treated groups. Conclusion: THP ex- hibited excellent activity of both growth inhibition and apoptosis induction on breast cancer cells. It' s recommended for further clinical study in breast cancer treatment.

关 键 词：表阿霉素 吡柔比星 生长抑制作用 人乳腺癌细胞 起效时间 药物诱导 体外杀伤作用 凋亡诱导 检测技术 存活率 

分 类 号：R737.9[医药卫生—肿瘤]