生物活性骨诱导再生膜的研制  

Experimental Study on the Fabrication of Bioactive Membrane for Inducing Bone Regeneration

作　　者：田卫东[1] 包崇云[1] 刘磊[1] 汤炜[1] 郑晓辉[1] 李声伟[1] 熊成东[2] 

机构地区：[1]四川大学华西口腔医学院,成都610041 [2]中国科学院成都有机化学所,成都610041

出　　处：《生物医学工程学杂志》2004年第5期844-847,共4页Journal of Biomedical Engineering

基　　金：四川省科技厅重点科研基金资助项目(G99004);教育部优秀青年教师教学科研奖励计划资助项目(B52003682)

摘　　要：研制一种具有生物活性的骨诱导再生膜。采用溶剂挥发法及冷冻干燥制备 PL A+胶原 +rh BMP- 2复合膜 ,对膜作力学性能测试 ,SEM表面特征观察 ,失重率及黏均分子量测定 ,体内外降解性评价 ;复合膜植入兔肌间隔 ,术后 1、2、3、6月取标本 ,光镜评价组织相容性和骨诱导活性。结果显示 PL A+胶原 +rh BMP- 2膜呈双面不对称并携带生物活性因子 ,拉伸强度 36 .4 MPa,断裂伸长率 2 .3% ;膜形态体内保持 3个月 ,体外 12个月或体内 6个月膜完全降解 ;复合膜随植入时间延长 ,局部炎症反应减轻 ,具有异位骨诱导性。由此可见 ,PL A+胶原 +rh BMP- 2膜是一种较理想的能控释生物活性因子的骨诱导活性再生膜。The aim of this study was to develop a bioactive membrane for inducing bone regeneration. The membrane was composed of polylactic acid, collagen, recombinant human bone morphogenetic protein-2(rhBMP-2). The PLA+collagen+rhBMP-2 membrane was fabricated by solvent-casting and cool-drying. The mechanic properties of this compound membrane were tested. The two surfaces of membrane were observed by SEM. Degradability of PLA was evaluated by SEM observation and molecular weight measure in vitro and in vivo. The compound membranes were implanted in rabbit muscles. The samples were obtained when animals were sacrificed at different periods: 2 weeks,1,2,3,6 months after surgery. The biodegradability and biocompatibility of the membrane were evaluated. The heterotopic bone inducing activity of BMP was identified. The results indicated that the strength at extension to failure of the compound membrane was 36.4MPa at 2.3% strain. The compound membrane was found bearing active factor on its coarse side, which can induce bone regeneration. After implantation in vivo, the membrane maintained the structure for three months and degraded in 6 months. Based on histological analysis, there was no obvious inflammation. Heterotopic bone was induced. We could conclude that the PLA+collagen+rhBMP-2 membrane is an absorbable compound membrane that possesses good biocompatibility, adequate mechanic properties and excellent property of bone induction. It could be applied as an ideal membrane for inducing bone regeneration.

关 键 词：骨诱导性 RHBMP-2 体内 胶原 植入 生物活性因子 局部炎症 PLA 复合膜 再生 

分 类 号：R318.08[医药卫生—生物医学工程] R683[医药卫生—基础医学]