干扰素对人结肠癌细胞HLA-ABC抗原表达的影响  

Regulative effect of interferon on HLA-ABC expression in human colon cancer.

作　　者：刘钢[1] 黄文广[1] 方泉 

机构地区：[1]华中科技大学同济医学院附属协和医院胃肠外科,武汉430022 [2]湖北省黄冈市第一人民医院普外科

出　　处：《华中医学杂志》2004年第5期309-311,F004,共4页Central China Medical Journal

摘　　要：目的　探讨干扰素 (IFN)对人结肠癌细胞HLA ABC抗原表达的影响。方法　采用免疫组化SP法和ELISA法分别检测不同剂量的α IFN和γ IFN刺激前后HLA ABC在四株人结肠癌细胞HCT 8、lovo、SW 4 80、Ls 174 T中的表达情况。结果　α IFN和γ IFN刺激之前 ,四株细胞中除lovo和Ls 174 T细胞不表达HLA ABC抗原 ,其它二株细胞均可不同程度表达HLA ABC抗原 ,刺激之后 ,对HLA ABC抗原的表达四株细胞系均有所增强 ,并呈现出干扰素依赖性及γ IFN的剂量依赖性 ,γ IFN的诱导作用强于α IFN。结论　干扰素可以上调人结肠癌细胞HLA ABC抗原的表达 。Objective To investigate the regulative effect of interferon (IFN) on the HLA-ABC expression in human colon cancer. Methods The expression of HLA-ABC in 4 colon cancer cell lines HCT-8、lovo、SW-480、Ls-174-T was detected by using immunohistochemical SP and ELISA methods before and after being stimulated by different doses of α-IFN or γ-IFN. Results No expression of HLA-ABC was detectable in lovo and Ls-174-T cells, but HCT-8 and SW-480 were positive for the HLA-ABC expression before stimulation with α-IFN or γ-IFN. HLA-ABC expression was enhanced on all of the 4 cell lines after stimulation, and it was correlated with IFN and the dose of γ-IFN. The effect of γ-IFN was more obvious than that of α-IFN. Conclusion IFN can enhance HLA-ABC expression in human colon cancer cells.

关 键 词：HLA-A 人结肠癌 干扰素 Γ-IFN 癌细胞 表达 抗原 刺激 诱导作用 细胞系 

分 类 号：R735.35[医药卫生—肿瘤] R446[医药卫生—临床医学]