外周血cTnI-mRNA监测病毒性心肌损伤价值的实验研究  

Value of circulating cTnI-mRNA detection for monitoring viral cardimyocyte injury

作　　者：朱健华[1] 姚登福[1] 高增栋[1] 吴玮[1] 吴信华[1] 邱历伟[1] 

机构地区：[1]南通医学院附属医院心内科,江苏南通226001

出　　处：《中国实验诊断学》2004年第5期465-468,共4页Chinese Journal of Laboratory Diagnosis

基　　金：江苏省自然科学基金项目资助 (NO .BK99160 )

摘　　要：目的　研究外周血心肌型肌钙蛋白 (cTnI) mRNA分析在监测病毒性心肌损伤发生发展和预后中的价值。方法　于BALB/c小鼠腹腔接种 1× 10 9TCID5 0柯萨奇病毒B3(CVB3)液诱发心肌损伤发生 ,分别在CVB3感染后第 3、6、9、12、15、18和 2 1天采集外周血后处死小鼠 ,留取鼠心脏作病理组织学检查 ,并以逆转录 聚合酶链反应 (RT PCR)分析心肌及外周血中cTnI基因的表达状态。结果　CBV3感染后 ,鼠心肌及循环血cTnI mRNA表达上调 ,与心肌细胞发生肿胀、炎性细胞浸润、核固缩及碎裂、变性、坏死、钙化等组织学改变相关。cTnI mRNA基因扩增 ,心肌组织全数阳性 ;外周血阳性率在对照组及感染后的第 3、6、9、12、15、18和 2 1天分别为 0 %、0 %、0 %、16 7%、4 0 %、71 4 %、83 3%和87 5 %。结论　病毒性心肌损伤时cTnI mRNA上调表达 ,循环血cTnIObjective To investigate the value of circulating cTnI-mRNA detection for monitoring myocardial injury development and prognosis. Methods Viral myocardial injury models in BALB/c mice were created by intraperitoneal inoculation with coxsackievirus B3(CVB3, 1×10 9 TCID50) for inducing myocardial injury. The total RNAs were extracted and cTnI-mRNA in mice cardiac tissues and circulating blood were amplified by nested-PCR during mice myocardial injury. Results In viral infected mice, the mRNA abundance for cTnI was upregulated in heart and circulating blood and associated with salient myocardial histopathologic features, including myocardial swell, inflammatory cell infiltration, pyhnosis, karyorrhexis, karyolysis, denaturalization, necrosis, and calcification. The cTnI-mRNA from infected-mice heart and circulating cardiac myocytes were analyzed by RT-PCR, the gene amplified positive fragment were found in all heart tissues. The incidence of cTnI-mRNA was 0%, 0%, 0%, 16 7%, 40%, 71 4%, 83 3%, and 87 5% in the controls, the 3 rd , 6 th , 9 th , 12 th , 15 th , 18 th , and 21 st day in circulating bloods from the infected mice, respectively. Conclusion The present data suggest that cTnI-mRNA expression is upregulated on viral myocardial injury, and detection of circulating cTnI-mRNA is a sensitivity genetic marker for monitoring myocardial injury development and prognosis.

关 键 词：心肌损伤 外周血 病毒性 感染后 RNA基因 监测 发生发展 CVB3 变性 表达 

分 类 号：R542.2[医药卫生—心血管疾病]