被动吸烟对神经管上皮中凋亡相关基因bcl-2和Bax表达的影响  

Effect of passive smoking on expression of Bcl-2 and Bax in embryonic neural epithelium

作　　者：于向民[1] 张建民[1] 闫凤霞[1] 王东[1] 潘晓亮[1] 李玲[1] 

机构地区：[1]青岛大学医学院,山东青岛266021

出　　处：《中国优生与遗传杂志》2004年第5期16-17,共2页Chinese Journal of Birth Health & Heredity

基　　金：山东省自然科学基金资助项目 (No :Y9813 0 42 )

摘　　要：目的　研究被动吸烟致畸中细胞凋亡的发生及其与凋亡相关基因Bcl- 2和Bax表达的关系。方法　采用自制的半封闭式被动吸烟染毒箱 ,用烟熏的方法建立金黄地鼠被动吸烟的致畸模型。用免疫组织化学和图象分析方法对鼠胚神经上皮Bcl- 2和Bax表达进行了定性、定位和定量检测。结果　 1.Bcl- 2和Bax正常分布于金黄地鼠胚胎神经上皮和周围间充质中 ,定位于核膜和胞浆 ,二者的表达量在孕 9天最高 ,之后逐渐下降。 2 .被动吸烟后各实验组胚胎神经管上皮Bcl- 2免疫组化染色强度均不同程度的低于对照组。Bax的表达均不同程度的高于对照组。结论　被动吸烟能抑制Bcl - 2的表达 ,促进Bax的表达 ,从而导致了过量细胞凋亡的产生。Objective: To study the passive smoking-induced teratogenesis on the expression of Bcl-2 and Bax. Methods: By using self-made passive smoking cage, the gold hamsters passive smoking model were established. Then the expression of Bcl-2 and Bax were investigated by immunohistochemistry and image analysis. Results: The Bcl-2 and Bax gene were expressed in the membrane of nuclei and cytoplasm in the neuroepithelial and mesenchymal cells, which reached the highest on the 9 day of pregnancy,and then decreased gradually. The expression of Bcl-2 was weaker in experimental group than control group, but the expression of Bax was more intense in experimental group than control group. Conclusion: Passive smoking can decrease the expression of Bcl-2 and increase the expression of Bax.

关 键 词：被动吸烟 bcl-2 Bax表达 凋亡相关基因 上皮 神经管 对照组 鼠胚 胚胎 核膜 

分 类 号：R363[医药卫生—病理学]