氟对人胎肝细胞细胞周期、凋亡及p53表达的影响  

Effects of Fluoride on Cell Cycle, Apoptosis and p53 Expression in Human Embryo Hepatocytes

作　　者：李晓燕[1] 禇启龙 夏涛[1] 刘俊玲[1] 陈学敏[1] 杨克敌[1] 王爱国[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系,武汉4300302 [2]湖北职业技术学院护理系,孝感432100

出　　处：《华中科技大学学报（医学版）》2004年第5期550-552,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目 (No .30 2 7115 5 )

摘　　要：目的　研究氟对人胎肝细胞 (L 0 2细胞 )细胞周期、凋亡及p5 3表达的影响。方法　体外培养的人胎肝细胞接触 4 0、 80、 16 0 μg/ml氟化钠 2 4h后 ,MTT法检测L 0 2细胞存活率 ,流式细胞术检测细胞凋亡率和周期构成比 ,Westernblot检测P5 3蛋白的表达水平。结果　高剂量氟染毒组细胞存活率显著低于对照组 (P <0 0 1) ;各染氟组S期细胞数与对照组相比均明显增多 (P <0 0 1) ,中、高剂量氟染毒组细胞凋亡率和p5 3蛋白表达量均显著高于对照组 (P <0 0 1)。结论　氟可使L 0 2细胞S期细胞数增多 ,诱导人胎肝细胞凋亡和 p5 3表达 ,并呈剂量 效应关系。Objective To investigate the effects of fluoride on cell cycle, apoptosis, and the level of p53 expression in human embryo hepatocytes (L-02 cells). Methods After the cultured human embryo hepatocytes (L-02 cells) were incubated with 40, 80, and 160 _g/ml sodium fluoride for 24 h in vitro, the survival rate of L-02 cells was measured by MTT method, apoptotic rate and cell cycle by flow cytometry, and the level of p53 expression detected by Western blot, respectively. Results The survival rate of L-02 cells treated by high dose of fluoride was significantly lower than that in the control group (P<0.01). Compared with the control group, the cell number in S phase was significantly increased in cells exposed to different doses of fluoride (P<0.01). The percentage of apoptotic rate and the level of p53 protein expression in middle and high doses of fluoride group were markedly higher than in control group (P<0.01). Conclusion Fluoride can increase the cell number in S phase, and induce apoptosis and p53 expression in L-02 cells in a dose-effect manner.

关 键 词：人胎肝细胞 P53表达 细胞凋亡率 对照组 细胞周期 高剂量 染毒 表达水平 体外培养 氟化钠 

分 类 号：R737[医药卫生—肿瘤] R512[医药卫生—临床医学]