协同刺激分子4-1BB和CD28对人外周血不同T细胞亚群的激活作用  

The role of costimulatory molecule 4-1BB and CD28 in activating of various T cell subsets from human peripheral blood

作　　者：吴俊英[1] 钱峰[1] 吕合作[1] 王伟[1] 李柏青[1] 

机构地区：[1]蚌埠医学院免疫学教研室,安徽蚌埠233003

出　　处：《细胞与分子免疫学杂志》2004年第5期533-536,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：安徽省教育厅自然科学研究计划项目资助 (No.2 0 0 1kj1 68)

摘　　要：目的 :探讨 4 1BB/ 4 1BBL协同刺激信号在CD4 + 和CD8+ T细胞活化、增殖中的作用 ,并与CD2 8/B7信号作比较。方法 :用抗CD3单抗 (mAb)刺激人外周血单个核细胞 (PBMC)。用阻断型抗 4 1BBLmAb和抗CD80mAb ,分别阻断 4 1BB/ 4 1BBL和CD2 8/B7 1协同刺激信号。利用流式细胞术 (FCM)检测CD4 + T细胞、CD8+ T细胞的增殖率、CD8/CD4T细胞的比值变化和细胞分泌IFN γ的情况。结果 :用抗 4 1BBLmAb和抗CD80mAb阻断相应的协同刺激途径后 ,CD4 + 和CD8+ T细胞的增殖和细胞分泌IFN γ的水平均明显下降。培养 8d,抗CD3mAb单独刺激组CD8/CD4T细胞的比值为 1.98± 0 .0 6 ;抗 4 1BBLmAb阻断组CD8/CD4T细胞的比值下降为 0 .96±0 .0 3;而在抗CD80mAb阻断组 ,其比值上升为 2 .6 9± 0 .16。结论 :4 1BB分子可在CD4 + T细胞和CD8+ T细胞的活化、增殖中提供协同刺激信号。 4 1BB分子所介导的协同刺激信号 ,在CD8+ T细胞活化及增殖中发挥了更为重要的作用 ;而CD2 8分子所介导的协同刺激信号则更有利于CD4 +AIM: To explore the role of 4-1BB molecule in the activation and proliferation of CD4 + T cells and CD8 + T cells and compare it with that of CD28 molecule. METHODS: Human peripheral blood mononuclear cells (PBMCs) were stimulated in vitro with anti-CD3 mAb. Anti-4-1BB mAb and anti-CD80 mAb were added so as to block respectively costimulatory signals of 4-1BB/4-1BBL and CD28/B7-1. Flow cytometry was used to detect the proliferation ratio of CD4 + T cells and CD8 + T cells and the secretion of IFN-γ. RESULTS: When 4-1BB/4-1BBL and CD28/B7-1 signals were blocked by anti-4-1BBL mAb and anti-CD80 mAb, the proliferative response and the secretion of IFN-γ by CD4 + T cells and CD8 + T cells were obviously decreased. In the group stimulated with anti-CD3 mAb only, the ratio of CD8/CD4 T cells was 1.98±0.06. In the groups blocked with anti-4-1BBL mAb and anti-CD80 mAb, the ratios were 0.96±0.03 and 2.69±0.16, respectively. CONCLUSION: 4-1BB molecule could provide a costimulatory signal for activation and proliferation of CD4 + T cells and CD8 + T cells. The costimulatory signal mediated by 4-1BB molecule could mainly induce the proliferative response of CD8 + T cells, while that mediated by CD28 molecule could induce the activation of CD4 + T cells mainly.

关 键 词：4-1BB CD28 增殖 活化 

分 类 号：R392.12[医药卫生—免疫学]