自体树突状细胞体外增强肿瘤患者引流淋巴结细胞抗肿瘤活性的作用  

Enhancing antitumor activity of tumor-draining lymph node cells by autologous dendritic cells

作　　者：彭正[1] 李荣[1] 李力[1] 张磊[1] 金婷[1] 

机构地区：[1]解放军总医院普通外科,北京100853

出　　处：《细胞与分子免疫学杂志》2004年第5期595-597,607,共4页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的 :探讨自体树突状细胞 (DC)体外对胃腺癌患者肿瘤引流区淋巴结 (TDLN)细胞抗肿瘤活性的增强作用。方法 :分离肿瘤患者的外周血单个核细胞 (PBMC)经IL 4、GM CSF和TNF α诱导其成熟 ,并以自体肿瘤冻融抗原预致敏 ,诱导其中的DC。另外 ,分离胃癌患者的TDLN细胞 ,在含有IL 2的培养体系中培养 ,并将TDLN细胞分为 3组 :(1)第 1组为肿瘤抗原致敏的DC加TDLN细胞 (D组 ) ;(2 )第 2组为冻融的肿瘤抗原加TDLN细胞 (Ag组 ) ;(3)第 3组不加DC及肿瘤抗原作为对照组 (L组 )。比较 3组TDLN细胞对胃腺癌细胞系KATO3和黑色素瘤细胞系A375的杀伤作用。结果 :D组对KATO3细胞系的杀伤作用明显优于Ag组与L组 ;而对A375细胞 ,各组细胞的杀伤作用没有明显差异。结论AIM: To enhance antitumor activity of tumor-draining lymph node(TDLN) cells by autologous dendritic cells(DCs) in vitro. METHODS: Peripheral blood mononuclear cells(PBMCs) were isolated from the patients with gastric adenocarcinoma and induced with GM-CSF, IL-4 and TNF-α to obtain mature DCs which were then sensitized by autologous tumor lysate. TDLN cells were isolated and purified from lymph nodes of the patients and incubated with IL-2. Then we designed three experiment groups: (1)first group: TDLN cells activated by sensitized DCs; (2) second group:TDLN cells activated by autologous tumor lysate; (3)third group: TDLN cells alone, which were used as control. Cytotoxic activity of TDLN cells to gastric adenocarcinoma cell line KATO3 and human melanoma cell line A375 were compared between 3 groups. RESULTS: The cytotoxicity of TDLN cells activated by sensitized DCs to KATO3 cells was significantly stronger than that of TDLN cells activated by autologous tumor lysate or TDLN cells alone. In contrast, for A375 cells, cytotoxic activities of three TDLN cells had no significant difference. CONCLUSION: Specific antitumor activity of TDLN cells can be enhanced significantly by activated autologous DCs.

关 键 词：自体 杀伤作用 肿瘤患者 体外 肿瘤抗原 黑色素瘤细胞 引流 胃腺癌细胞 抗肿瘤活性 培养体系 

分 类 号：R735[医药卫生—肿瘤] R730[医药卫生—临床医学]