低浓度Pb^(2+)、Cd^(2+)对鲫鱼肝脏组织中HSP70诱导的影响  

Effects of Pb^(2+) and Cd^(2+) on the induction of HSP70 in the fish liver

作　　者：沈骅[1] 王晓蓉[1] 张景飞[1] 刘晓华[1] 赵永娟[2] 

机构地区：[1]南京大学环境学院污染控制与资源化研究国家重点实验室,江苏南京210093 [2]南京大学生物化学系医药生物技术国家重点实验室,江苏南京210093

出　　处：《环境污染与防治》2004年第4期244-246,共3页Environmental Pollution & Control

基　　金：国家自然科学基金重点资助项目 ( No.2 0 2 3 70 10 )

摘　　要：以鲫鱼 ( Carassius auratus)作为实验对象 ,经过 40 d Pb2 +、Cd2 +不同浓度的暴露后 ,运用 SDS-PAGE和 Western Blot-ting方法检测鱼肝脏组织内应激蛋白 HSP70的诱导表达情况。结果表明 ,在实验浓度下 ,与对照组相比 ,Pb2 + 、Cd2 + 对鱼肝脏内HSP70有显著的诱导 ( P<0 .0 5 ) ,但在 Cd2 +浓度为 0 .2 mg/ L 有停止表达 HSP70的现象 ,可能是由于浓度过高造成了组织病理损伤而破坏了诱导表达机制。实验还发现 ,在实验浓度低于国家渔业用水标准时 ,HSP70仍然表现为明显诱导 ( P<0 .0 5 ) ,充分说明运用分子生物学指标要比传统的环境检测指标敏感 。The effects of lead (Pb\+\{2+\}) and cadmium (Cd\+\{2+\}) on the induction of HSP70 in the fish liver were studied .After the fish \%(Carassius auratus)\% were exposed at the experimental concentration for forty days,the SDS PAGE and Western Blotting technique were used to detect the HSP70 in the fish liver. That the test groups exposed to the lead (Pb\+\{2+\}) and cadmium (Cd\+\{2+\}) all showed a significant increase in HSP70 as a function of exposure (\%P\%<0.05),but at the concentration of 0.1 mg/L ,it was found that the induction of HSP70 has been stopped perhaps for the high concentration of the toxic heavy metal cadmium (Cd\+\{2+\}). It was also found that the fish exposed to the concentration of Pb\+\{2+\} and Cd\+\{2+\} which below the national fishery water quality standard also have a significant increase in HSP70. It means that the low concentration of the heavy metal also can harm the fish body. So HSP70 as a environmental biomarker can be a more sensitive and rapid indicator than conventional end points such as the growth death.

关 键 词：HSP70 诱导 肝脏组织 实验 鱼肝 暴露后 对照组 CD^2+ PB^2+ 污染物 

分 类 号：X174[环境科学与工程—环境科学]