P16基因在口腔鳞癌中的表达及意义  

EXPRESSION OF P16 GENE IN ORAL SQUAMOUS CELL CARCINOMA AND ITS SIGNIFICANCE

作　　者：张韬[1] 杜德顺[1] 李鹏[1] 肖镜莲[1] 赖钦声[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院口腔科,北京100730

出　　处：《北京口腔医学》2004年第3期148-150,162,共4页Beijing Journal of Stomatology

摘　　要：目的 :研究口腔鳞癌中P1 6基因的蛋白及mRNA表达情况 ,探讨其在口腔鳞癌发生发展中的作用及意义。方法 :采用免疫组化SP法及原位杂交法检测 71例口腔鳞癌中P1 6蛋白的表达和 2 0例口腔鳞癌中P1 6mRNA表达情况并分析其与临床病理参数的关系。结果 :P1 6蛋白失表达率在口腔癌中为 4 9 30 % (35 /71 ) ,显著高于癌旁黏膜组 (P <0 0 1 )。但其表达与患者的年龄、性别、肿瘤部位、肿瘤大小、临床分期、病理分级、颈淋巴结转移及预后无关。原位杂交结果与免疫组化结果基本一致。结论 :口腔鳞癌中存在较高频率的P1 6蛋白失表达 ,P1Objective:To study the expression of P16 protein and mRNA in oral squamous cell carcinoma (OSCC) and to evaluate its role in carcinogenesis and progression of oral squamous cell carcinoma.Methods:The expression of P16 protein in 71 cases of OSCC was examined by immunohistochemical technique (SP),and the expression of P16 mRNA in 20 cases of OSCC studied by in situ hybridization,and its relation to clinicopathological parameter was also analyzed.Results:The loss expression rate of P16 protein was 49 30% (35/71).There was significant difference between OSCC and adjacent tumor-free oral mucosa.But P16 protein had no correlation with patients’ age,sex,tumor site,size,pathology grade,clinical stage,lymph node metastasis and survival.Conclusion:There was a high frequency of P16 protein expresssion loss in OSCC.P16 gene may be involoved in carcinogenesis and progression of OSCC.

关 键 词：口腔鳞癌 mRNA表达 P16基因 P16蛋白 癌发生 癌旁黏膜 白及 表达率 颈淋巴结转移 口腔癌 

分 类 号：R739.81[医药卫生—肿瘤]