兔肾VX_2肿瘤模型超声显像与肿瘤增殖活性及侵袭性的关系  

Ultrasonographic observations on proliferative activity and invasiveness of rabbit renal VX_2 tumor

作　　者：王文[1] 段云友[1] 曹铁生[1] 吕发勤[1] 李莹莹[1] 袁丽君[1] 赵柏山[1] 

机构地区：[1]第四军医大学唐都医院超声科,西安710038

出　　处：《中华超声影像学杂志》2004年第8期619-622,共4页Chinese Journal of Ultrasonography

基　　金：陕西省科学基金资助 (2 0 0 1SM47)

摘　　要：目的　探讨兔肾VX2 肿瘤超声观测指标与其增殖活性及侵袭性的关系。方法　建立 5 6只兔肾VX2 肿瘤模型 ,种植 3周后应用二次谐波和声像图定量技术检测癌灶大小、回声强度、边缘回声及能量面积比 ,并与肿瘤的组织病理学、流式细胞仪测定的增殖指数 (PI)及倍体细胞率 (PCR )相比较。结果兔肾VX2 肿瘤的PI及PCR与肿瘤边缘回声无明显相关 ,而与肿瘤大小、内部回声强度及血流丰富程度有一定关系。结论　超声观测指标可用于推测肾癌的增殖活性及侵袭性 。Objective To explore the correlation between the ultrasonographic parameters describing the tumor and proliferative activity, invasiveness of the rabbit renal VX 2 tumor. Methods Animal models were established in 56 rabbits,in which left kidneys were transplanted with VX 2 tumor. The second harmonic imaging and video-density quantitative determination were applied to observe the size, echo-intensity and edge echo-pattern of the rabbit renal VX 2 tumor. The findings were compared with those of histopathologic section and DNA analysis calculated with the flow cytometer. Results The number of tumor cell with higher proliferative index(PI) and ploidy cell rate(PCR) increased with the tumor size. The tumors of hyperecho and more blood flow with obvious necrosis tissue, fibrotic lesion and blood sinusoid got higher PI and PCR than the tumors of hypoecho and less blood flow, while there was no statistic significant correlation between the echoic patterns of the tumor edge and DNA analysis.Conclusions The ultrasonographic parameters describing the tumor, such as the size, echo-intensity and blood flow, can be used to evaluate the proliferative activity and invasiveness of the renal tumor.

关 键 词：兔 肾VX2肿瘤 动物模型 超声显像 肿瘤增殖活性 肿瘤侵袭性 

分 类 号：R737.11[医药卫生—肿瘤]