羟基磷灰石涂层植入体在兔的去卵巢骨质疏松模型中的生物学特性研究  

Biological characterization of hydroxylapatite-coated implants in model of osteoporosis induced by ovariectomy

作　　者：杨明亮[1] 罗先正[1] 任素梅[1] 郭艾[1] 李强[1] 唐海[1] 刘正秋[1] 

机构地区：[1]北京市中国康复研究中心脊柱外科,北京友谊医院骨科

出　　处：《中国骨质疏松杂志》1997年第2期7-14,共8页Chinese Journal of Osteoporosis

摘　　要：本实验旨在通过建立兔的去卵巢骨质疏松模型，观察羟基磷灰石涂层植入体在此类异常骨床中的生物学特性。选用健康雌性家兔，随机分为Ａ、Ｂ两组，Ｂ组在行植入体术前４周切除双侧卵巢，Ａ组模拟切除双侧卵巢。烧结的ＨＡ涂层钛金属植入体及单纯钛金属植入体被分别植入动物骨胶远端，植入体周骨床被划分为四区，远、中、近１／３段分别为１、２、３区，近１／３段游离端为４区。Ｂ组动物在去卵巢后８周，雌二醇下降为术前的２３．３％，股骨远端的骨密度在去卵巢术后４周较对照组低６．５％，有显著性差异（Ｐ＜０．０５）。Ａ、Ｂ两组中均以２、４区为骨密度高值区，ＨＡ涂层能促进植入体周的骨形成，骨组织内生在４～８周达高峰，８周后有再吸收趋势，ＨＡ涂层能明显促进植入体的生物固定强度，去卵巢引起的低骨床对植入体生物固定强度有削弱的趋势。Abstract: In the model of postmenopausal osteoporosis of rabbits induced by ovariectomy,the biological profile of HA-coated implants and Ti implants was investigated. The animals were divided into group A and group B. The group B had been ovariectomied before 4 weeks,and the group A was used as control. The two types of implants,the cylindrical sintering HA-coated Ti implants and Ti implants were 3 mm in diameter with overall length of 30 mm,were inserted into the distal femurs of all rabbits,one femur receiving a HA-coated implant and the other one with a Ti implant serving as a control. The implant was divided into three sections. The distal section was located in cancellous bone bed and fixed by press-fit.The middle in tinea epiphysis surrounded by 0-2mm gap,and the proximal surrounded by bone marrow tissue. The bone bed of the implant was also divided into three zones,zone 1,zone 2,and zone 3 from the distal to proximal. Another interested zone 4 was located in the top of proximal implant. Bone mineral density in every zone was analyzed by DPX-L densitometer. After follow-up periods of four,eight,twelve weeks,the rabbits were sacrificed. The femurs were used for histologic examination,mechanical push-out testing and radiography. The estrogen in group B was significantly lower by 76. 7%than that of pre-ovariectomy. The lumbar spine (L2-L4) bone mineral density in group B declined at a rate of 6. 3% for the first 4 weeks of observation as compared with the group A and by 3. 0% for the second 4 weeks,In the distal femurs,the BMD declined at a rate of 6. 5 % for the first 4 weeks. The bone loss in the bed of implants varied with zones. The BMD in zone 4 and zone 2 increased markedly for the period of 4-8 week follow-up. and decreased after the 8 week follow-up. The HA coating increased BMD. The implant-bone interface shear strength of the HA-coated implants in group B is 6. 5 times than that of Ti implants. and 7. 5 times in group A (P<0. 05),although the differences in shear strength values between group A an

关 键 词：植入体 去卵巢 HA涂层 羟基磷灰石涂层 双侧卵巢 切除 骨密度 术前 骨质疏松模型 生物固定 

分 类 号：R783[医药卫生—口腔医学] R318[医药卫生—临床医学]