VDR基因型分布及其与骨矿含量的关系  

Vitamin D receptor gene and bone mineral content

作　　者：闫丽娅[1] 王晓红[1] 张卉[1] 侯菊倩[1] 韩延柏[1] 

机构地区：[1]沈阳医学院预防医学系

出　　处：《中国骨质疏松杂志》1997年第2期19-21,共3页Chinese Journal of Osteoporosis

摘　　要：近年来，在骨质疏松研究领域，维生素Ｄ受体基因（ＶＤＲ）与骨量及骨代谢的研究受到许多国外学者的重视。我们利用国际合作的机会，对９６名沈阳妇女ＶＤＲ基因进行了分析。研究对象来自于一项正在进行的骨代谢影响因素研究课题。采用标准方法（Ｎｕｃｌｅｏｎｋｉｔ，ｓｃｏｔｌａｂ，ＵＫ）从白细胞中提取ＤＮＡ，以聚合酶链反应（ＰＣＲ）来扩增特定基因段。用ＢｓａＭＩ限制性内切酶消化ＰＣＲ产物，以２％琼脂糖电泳分离判定ＶＤＲ基因型。使用ＤＰＸ－Ｌ（ＬｕｎａｒＵＳＡ）骨矿测定仪测定研究对象的腰椎（Ｌ２～４）及髋部骨矿含量（ＢＭＣ）。结果：与其他国家人群相比，该人群ＶＤＲ基因的分布状态不同。ＶＤＲ基因的ｂｂ型占总数的９１．７％；Ｂｂ型仅占８．３％，而且ＢＢ基因型完全不存在。在青年妇女中发现ｂｂ基因型组股骨颈ＢＭＣ高于Ｂｂ型组股骨颈ＢＭＣ（Ｐ＝０．０２７）。老年妇女ｂｂ基因型组股骨颈ＢＭＣ也高于Ｂｂ型组，但差异无统计学意义，没有发现其他部位ＢＭＣ与ＶＤＲ基因型有关。The associations between alleies of vitamin D receptor(VDR) and bone mineral density have been reported in recent years. We analysed the genotypes of VDR and examined the relationships between VDR genotypes and bone mineral content (BMC)in 96 healthy women in Shenyang. The subjects were recruited as part of detailed study on bone metabolism in Shenyang. DNA was extracted using a standard method (Nucleon Kit,ScotLab,UK)from leukocytes. The VDR gene was amplified by using polymerase chain reaction(PCR). PCR products were restricted by BsaMI restriction enzyme. Restriction fragments were separated and determined in 2 % agarose. BMC of the lumbar spine (L2~4)and hip were measured by DPX-L densitometry. We found that the frequency of bb genotype was counted for 91. 7 %and Bb for 8. 3% in Shenyang women. No BB genotype was found in this population. A high BMC of femoral neck was associated with the bb genotype in young women (P= 0. 027). Statistically not significant high BMC of femoral neck was also found in old women with bb genotype. There were no relationships of VDR genotypes to BMC at other sites.

关 键 词：骨矿含量 基因型分布 股骨颈 骨代谢 人群 BMC B基因 VDR 琼脂糖电泳 维生素D受体基因 

分 类 号：R681[医药卫生—骨科学] R394[医药卫生—外科学]