IL-1B及IL-1RN基因多态性与胃癌易感性的关系  

Association between polymorphisms of interleukin-1 B and interleukin-1 receptor antagonist genes and host susceptibility to gastric cancer

作　　者：杨婕[1] 胡志斌[1] 徐耀初[1] 沈靖[1] 钮菊英[1] 胡旭[2] 郭剑涛[3] 沈洪兵[1] 

机构地区：[1]南京医科大学公共卫生学院流行病与生物统计学系,江苏省南京市210029 [2]淮安市疾病预防控制中心,江苏省淮安市223200 [3]金坛市疾病预防控制中心,江苏省金坛市213200

出　　处：《世界华人消化杂志》2004年第8期1769-1773,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金项目;No.30170827;30271148~~

摘　　要：目的:获取白介素1B(IL-1B)基因启动子区域C-31T,C- 511T,C+3954T位点单核苷酸多态性和白介素1受体拮抗剂(IL-1RN)基因串联重复多态性在江苏高发区人群胃癌患者与健康对照中的频率分布资料,初步探讨其各基因型与胃癌易感性的关系. 方法:采用人群为基础的病例对照研究方法,共收集285 例胃癌患者与265例性别、年龄和地区频数匹配的对照外周血标本,同时调查流行病学资料.从外周血提取基因组DNA,以聚合酶链式反应-限制性片段长度多态性(PCR- RFLP)方法对各位点进行基因分型. 结果:IL-1B基因-31位点3种基因型CC,CT,TT在胃癌病例组中频率分别为21.8%,48.6%和29.6%;在对照组中为27.9%,48.8%和23.3%(P=0.203).相似的,-511 位点TT,CT,CC基因型在病例与对照组中频率分别为18.2%,56.5%,25.3%和24.9%,52.5%,22.3%(P=0.184). 在调整年龄、性别等因素后,携带-31TT基因型者比CC型胃癌发生的危险性增加75%(P=1.75,95%CI=1.08-2.85); 携带-511CC基因型者比TT型危险性增加63%(P=1.63, 95%CI=0.98-2.73).分层分析表明,这一危险性在H pylori 感染阳性人群中更为显著.IL-1RN和IL-1B+3954位点各基因型在病例与对照组中的分布没有显著性差异. 结论:IL-1B基因启动子区域-31,-511位点多态性可能与中国人群胃癌易感性相关,尤其在H pylori感染人群中.AIM: To explore the genotype frequencies of polymorphisms in the interleukin-1B (IL-1B) gene promoter region -31, -511 and +3 954 loci and interleukin-1 receptor antagonist gene (IL-1RN) variable number of tandem repeat (VNTR) in gastric cancer cases and cancer-free controls in a Chinese population, and to evaluate the association between polymorphisms of IL-1B and IL-1RN genes and host susceptibility to gastric cancer. METHODS: Genotypes were determined by a modified PCR-restriction fragment length polymorphism (PCR-RFLP) approach in 285 histologically-confirmed gastric cancer patients and 265 age-, sex- and residence area frequency-matched cancer-free controls in a Chinese population. RESULTS: The genotype frequencies of IL-1B C-31T polymorphism were 21.8% for CC, 48.6% for CT and 29.6% for TT among gastric cancer patients, and 27.9%, 48.8% and 23.3% among control subjects (P =0.203). Similarly, the genotype frequencies of IL-1B T-511C polymorphisms were 18.2% for TT, 56.5% for CT, 25.3% for CC among pa- tients and 24.9%, 52.5%, 22.3% among controls (P =0.184). Multivariate logistic regression analyses revealed that the risks associated with the IL-1B variant genotypes were 1.75 (95%CI =1.08-2.85) for -31TT and 1.63 (95% CI = 0.98-2.73) for -511CC, respectively, compared with their wild-type homozygotes. Furthermore, we found a greater increased risk in individuals with both H pylori nfection and the variant genotypes (adjusted OR 2.14, 95% CI= 1.13-4.06 for -31TT; adjusted OR 2.00, 95% CI= 1.02-3.89 for -511CC), which was consistent with the biological effects of IL-1β. No significant association was observed between polymorphisms of IL-1RN gene and IL-1B gene +3 954 locus and gastric cancer risk. CONCLUSION: The two IL-1B polymorphisms within promoter region may contribute to the risk of developing gastric cancer in our Chinese population, especially in individuals with H pylori nfection.

关 键 词：胃癌 易感性 人群 IL-1RN 对照组 基因型 白介素1 结论 获取 CC 

分 类 号：R735.2[医药卫生—肿瘤] R394[医药卫生—临床医学]