广西人上消化道疾病患者幽门螺杆菌cagA基因检测及其临床意义  

Detection of Helicobacter pylori cagA gene in patients with digestive diseases in Guangxi and its clinical significance

作　　者：黄赞松[1] 唐国都[2] 王超[1] 李素艳[2] 姜海行[2] 

机构地区：[1]右江民族医学院附属医院消化内科,广西壮族自治区百色市533000 [2]广西医科大学第一附属医院消化内科,广西壮族自治区南宁市530021

出　　处：《世界华人消化杂志》2004年第8期1831-1834,共4页World Chinese Journal of Digestology

摘　　要：目的:研究广西人上消化道疾病患者幽门螺杆菌(H pylori)细胞毒素相关蛋白(cagA)基因的阳性率,从而明确广西人群感染H pylori菌株的毒力状况. 方法:在广西南宁市几家大医院的慢性胃炎(CG)、消化性溃疡(PU)患者胃黏膜中分离出45株H pylori,用特异性引物扩增H pylori cagA基因的297 bp片段,经聚合酶链反应(PCR)进行检测.同时收集患者临床和胃镜诊断资料,分析H pylori cagA基因与胃十二指肠疾病的关系. 结果:在45株H pylori中有84.4%(38/45)的菌株含有cagA基因,其中PU患者感染菌株cagA基因阳性率为76.0%(19/25), CG患者cagA基因阳性率为95.0%(19/20),二者比较差异无显著性(X2=3.054,P>0.05),各年龄组患者感染H pylori 菌株cagA基因阳性率差异无显著性(P>0.05),而女性患者阳性率(100.0%)显著高于男性(73.1%,P<0.01). 结论:广西人上消化道疾病患者H pylori cagA基因阳性菌株感染率较高;PU患者感染菌株cagA基因阳性率低于CG 患者;女性患者感染H pylori菌株cagA基因阳性率明显高于男性;但各年龄组患者阳性率无差异.AIM: To study the positive rate of Helicobacter pylori(H pylori) cagA gene of strains and the virulence of H pylori infecting patients in Guangxi, China. METHODS: H pylori strains were isolated in gastric biopsy specimens from the patients with chronic gastritis and peptic ulcer in Nanning area of Guangxi. The sequence of D008/R008 primers were used to amplify 297 bp fragment of cagA gene and PCR were used to detect the frequency of cagA gene in the 45 H pylori isolates. RESULTS: The positive rate of cagA gene in H pylori strains was 84.4% (38/45). The positive rate of cagA gene in peptic ulcer (PU) patients was 76.0% (19/25), which was lower than that in chronic gastritis (CG) patients (95.0%, 19/20), but there was no significant difference (P >0.05). The positive rate of cagA gene was higher in female patients than that in male patients (100.0% vs73.1%,P<0.01). The positive rate of cagA gene in different age groups was no significant difference (P>0.05). CONCLUSION: The positive rate of cagA gene in H pylori strains among patients in Guangxi is high, and the positive rate is lower in PL) patients than that in CG patients, and higher in male than female patients. But there is no significant difference in different age groups.

关 键 词：女性患者 CAGA基因 阳性率 上消化道疾病 幽门螺杆菌 感染菌 菌株 年龄组 结论 差异 

分 类 号：R57[医药卫生—消化系统]