大鼠EAMG模型眼外肌下调差异基因的初步筛选  

Screening Down-regulated Differential Genes of Extraocular Muscle in Rat's Model of Experimental Autoimmune Myasthenia Gravis

作　　者：刘睿[1,2] 李柱一[1] 王桂平[1] 许汉鹏[3] 施新猷[4] 鞠躬[3] 

机构地区：[1]第四军医大学唐都医院神经内科 [2]第四军医大学神经科学研究所710032 [3]第四军医大学神经科学研究所 [4]第四军医大学药物研究所

出　　处：《中国临床神经科学》2004年第3期223-225,233,共4页Chinese Journal of Clinical Neurosciences

基　　金：国家自然科学基金资助项目 (30 2 70 4 85)

摘　　要：目的 :通过研究大鼠实验性自身免疫性重症肌无力 (EAMG)模型眼外肌存在差异表达的基因 ,探讨重症肌无力患者眼外肌无力的可能机制。方法 :将乙酰胆碱受体单抗mAb3 5和生理盐水分别给予实验组和对照组F3 44大鼠腹腔注射 ,48h后取新鲜眼外肌提取总RNA ,经反转录后获得标记cDNA探针 ,将其与BioStarR 40S表达谱基因芯片杂交 ,结果由扫描仪扫描并用软件进行分析统计。结果 :共筛选出 5 5条表达差异的基因 ,其中 3 0条表达降低。结论 :多种基因参与了重症肌无力眼外肌无力过程 ,差异基因表达的检测结果可为深入研究重症肌无力患者眼外肌无力机制提供新思路。Aim:To seek for the pathogenesis of the extraocular muscle involvement in myasthenia gravis (MG) by analyzing the gene expression of the extraocular muscles in experimental autoimmune myasthenia gravis(EAMG) models in rats.Methods:To inject monoclonal antibody (mAb35) directed towards the acetylcholine receptor to the experimental rats and inject the control with saline. The total RNAs were isolated 48 hours later from fresh extraocular muscle and then they were reversely transcribed to cDNA which were hybridized to gene chip of BioStarR-40S. The gene chip was scanned for the fluorescent signals and the differential genes were analyzed with the software. Results:Fifty five differential genes were screened out, among these genes, down-regulated genes were thirty. Conclusion:Many genes are involved in the onset of extraocular muscle weakness in myasthenia gravis.

关 键 词：眼外肌 大鼠 重症肌无力 EAMG模型 患者 差异基因 初步筛选 反转录 表达谱 总RNA 

分 类 号：R746.1[医药卫生—神经病学与精神病学] R777[医药卫生—临床医学]