深圳东门市场野生动物SARS病毒的监测  

Surveillance of SARS Coronavirus among wild animal sold in Dongmen Market in Shenzhen City

作　　者：何雅青[1] 杨洪[1] 赵锦[1] 刘涛[1] 刘建军[1] 房师松[1] 何建凡[1] 周丽[1] 程小雯[1] 吕星[1] 管轶[2] 郑伯健[2] 周俊安 张丹 刘小立[1] 庄志雄[1] 

机构地区：[1]广东省深圳市疾病预防控制中心,广东深圳518020 [2]香港大学微生物学系 [3]深圳市卫生局

出　　处：《疾病监测》2004年第8期287-290,共4页Disease Surveillance

摘　　要：目的　对深圳东门市场野生动物标本中SARS病毒进行监测 ,了解动物携带SARS病毒的情况。方法　对 2 0 0 3年 5月 7日至 12月在东门市场采集的果子狸、貉、鼬獾、猪獾和狗獾等 5种动物标本 ,进行病毒分离鉴定及RT -PCR和SARS荧光定量PCR检测。结果　 5月首先从 4份果子狸和 1份貉标本中分离到SARS病毒。 5月至 12月的 12 1份动物标本中共有 5 5份SARS病毒阳性标本 ,阳性率为 4 5 4 5 %。 5月份、10月份、11月份和 12月份野生动物标本中SARS阳性率分别为 5 0 %、83 33%、34 78%和 4 4 87% ,其中 ,果子狸的SARS病毒携带率为 90 %。结论　东门市场售卖的果子狸可能是携带SARS病毒的重要载体之一 ,在SARS的传播中可能起着重要的作用。Objective To survey SARS Coronavirus among wild animals sold in Dongmen Market and to learn the carried rate of SARS Coronavirus.Methods Samples of civet cat,raccoon-dog,melogalemoschata,arctonyx collaris and meles were collected from Dongmen Market from 7th May to December in 2003.SARS-Cov were isolated,and were detect by fluorescence PCR and RT-PCR.Results SARS-Cov were isolated from four civet cats and one raccoon-dog in May.In 121 animal samples,55 were SARS-Cov positive from May to December,the positive rate was 45.45%. In May,October,November and December,the positve rate of SARS-Cov for wild animal samples were 50%,83.33%,34.78% and 44.87%,respectively. The SARS-Cov carried rate of civet cats was 90%.Conclusion Civet cat sold in Dongmen market was probably one of significant carriers for SARS-Cov,and was playing an important role in SARS epidemic.

关 键 词：SARS病毒 阳性标本 携带率 监测 阳性率 果子狸 荧光定量PCR检测 月份 野生动物 狗獾 

分 类 号：S852.65[农业科学—基础兽医学]