寡核苷酸芯片技术在胰腺癌诊断中的应用  

Application of oligonucleotide microarray in the diagnosis of pancreatic carcinoma

作　　者：徐迎新[1] 刘哲[2] 徐勤生[1] 宋旭华[1] 陈琳洁 冯玉泉[2] 

机构地区：[1]解放军总医院普通外科研究所,北京100853 [2]解放军总医院肝胆外科

出　　处：《中华实验外科杂志》2004年第9期1085-1086,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目(30070736)

摘　　要：目的 探讨基因突变寡核苷酸芯片技术检测K-ras基因与p53基因突变在胰腺癌诊断中的价值。方法应用芯片技术检测25例胰腺癌和5例慢性胰腺炎患者外周血浆游离DNA和组织标本中K-ras基因与p53基因突变。结果胰腺癌病人外周血血浆游离DNA中K-ras基因突变率为52.0％,p53基因突变率为20.0％;联合检测阳性率为60.0％,肿瘤组织标本中K-ras基因突变率为76.0％,p53基因突变率为32.0％,联合检测阳性率为92.0％。结论寡核苷酸芯片技术能够为胰腺癌的相关基因分析提供可靠的数据,检测K-ras基因突变有助于胰腺癌早期诊断,联合检测p53基因突变可提高诊断的敏感性和特异性。Objective To discuss the value of the detection of mutations in K-ras gene and p53 gene in the diagnosis of pancreatic carcinoma. Methods Twenty-five patients with pancreatic carcinoma and 5 patients with chronic pancreatitis were selected. Mutations in K-ras gene at codon 12 and p53 gene in the cell-free DNA fragments in plasma and genomic DNA from tumor tissue were analyzed using the technique of oligonucleotide microarray. Results The positive rate of K-ras and p53 gene point mutation in plasma was 52.0% and 20.0% respectively. The combined positive rate in plasma was 60.0 % . In tu-mor tissue, the positive rate of K-ras and p53 gene point mutation and the combined positive rate was 76.0 % , 32.0 % and 92.0 % respectively. Conclusion The oligonucleotide microarray is a powerful technique for analysis of associated genes in pancreas carcinoma. The detection of K-ras gene mutation is in favor of the early diagnosis of pancreatic carcinoma. Simultaneous analysis of K-ras and p53 mutation is suggested to enhence the genetic diagnosis of pancreatic carcinoma.

关 键 词：胰腺癌 诊断 P53基因突变 K-RAS基因突变 寡核苷酸芯片 联合检测 游离DNA 标本 特异性 相关基因 

分 类 号：R735.9[医药卫生—肿瘤]