增生性瘢痕内3种成纤维细胞生长因子及其受体基因的表达  

Gene expression of fibroblast growth factors and their receptors in proliferative and mature hyper-trophic scars

作　　者：陈伟[1] 付小兵[1] 周岗[1] 姜笃银[1] 孙同柱[1] 盛志勇[1] 

机构地区：[1]解放军304医院全军创伤修复重点实验室,北京100037

出　　处：《中华实验外科杂志》2004年第9期1111-1113,共3页Chinese Journal of Experimental Surgery

基　　金：国家重点基础研究发展规划项目(G1999054204);国家自然科学基金重点项目(30230370)

摘　　要：目的 探讨成纤维细胞生长因子(FGF)-7、酸性FGF(aFGF)、碱性FGF(bFGF)及其受体(FGFR1、FGFR2)在不同形成时期的增生性瘢痕中的基因表达。方法用病理学技术检测增生性瘢痕和正常皮肤的结构特征后,提取16例不同发生时期的增生性瘢痕和8例正常皮肤的总RNA后,分离mRNA,用逆转录-聚合酶链反应(RT-PCR)方法检测这5种基因在不同组织中的表达。结果在正常皮肤中,FGF-7,bFGF,FGFR1和FGFR2基因表达水平较低,而在增殖期的瘢痕中,这4种基因转录本的灰密度值分别为正常皮肤的2.1、2.1、3.6和2.8倍,基因表达显著增强(P<0.05);在成熟期的瘢痕中,FGF-7,FGFR1和FGFR2基因的表达量都低于增殖期的瘢痕,而bFGF仍保持高水平的基因表达。在正常皮肤和增殖期的瘢痕中,aFGF基因呈低水平表达,而在成熟期的瘢痕中aFGF基因表达明显增强(P<0.05)。结论FGF-7、bFGF及其受体基因表达升高可能是增生性瘢痕形成的机制之一,而FGF-7、FGFR1和FGFR2基因表达降低,aFGF表达增强可能与增生性瘢痕达到相对稳定的成熟期有关。Objective To investigate gene expression of fibroblast growth factor-7 (FGF-7), acid fibroblast growth factor (aFGF), basic fibroblast growth factor (bFGF) and their 2 receptors (FGFR1 and FGFR2) in normal skin versus hypertrophic scars underlying their potential biological significances. Methods After total RNA was isolated from 16 specimens of hypertrophic scars and 8 specimens of normal skins, they was purified to mRNA. The gene expression of FGF-7, aFGF, bFGF, FGFR1 and FGFR2 was detected by using reverse transcription-polymerase chain reaction (RT-PCR). Results In normal skin, the levels of the gene expression of FGF-7, bFGF, FGFR1 and FGFR2 were lower. In proliferative hypertrophic scars, the contents of these 4 gene transcripts were 2.1,2.1,3.6 and 2.8 times of those of normal skin, respectively. In mature scars, the gene expression of FGF-7, FGFR1 and FGFR2 was obviously decreased as compared with that in proliferative scars, while the bFGF was highly expressed, transcript in hypertrophic scars with various periods after wound were no substantial change ( P > 0. 05 ) . The expression of aFGF transcript in normal skin and proliferative scars was down-regulated, while it was strongly expressed in mature scar. Conclusion The activation of gene expression of FGF-7, bFGF and receptors might be one of the mechanisms regulating cicatrization of hypertrophic scars, while the decreased levels of gene expression of FGF-7, FGFR1 and FGFR2 in mature scars and the increased expression of aFGF might be associated with hypertrophic scars reaching steady stage.

关 键 词：FGFR1 增生性瘢痕 正常皮肤 基因表达水平 成纤维细胞生长因子 增殖期 受体基因 表达量 总RNA 基因转录 

分 类 号：R363[医药卫生—病理学]