牛珀至宝微丸对局灶性脑缺血再灌注后神经前体细胞的影响(英文)  

Effect of niupo zhibao weiwan on neural precursor cells after focal cerebral ischemic reperfusion

作　　者：廖欣[1] 杜少辉[1] 陈东风[2] 张宜 黎晖[2] 魏志军[1] 

机构地区：[1]深圳市中医院内科,广东省深圳市518033 [2]广州中医药大学解剖教研室,广东省广州市510405 [3]深圳市沙河医院内科,广东省深圳市518033

出　　处：《中国临床康复》2004年第28期6253-6255,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(39970914)~~

摘　　要：背景:成年个体海马和室管膜下层细胞具有分裂产生新生神经元的能力,并分离了能不断分裂增殖且具有多种分化潜能的细胞群,即神经干细胞,但因分离、纯化十分困难,分离过程易造成神经细胞损伤,影响其活性而易致凋亡,开发中药诱导体内神经干细胞的增殖作用具有重要的临床意义。目的:观察牛珀至宝微丸对局灶性脑缺血再灌注后神经前体细胞的影响,探讨其治疗缺血性脑血管病的机制。设计:随机对照单盲的实验研究。地点和对象:实验地点:广州中医药大学基础学院神经解剖实验室,研究对象为纯种SD大鼠24只。干预:采用大脑中动脉线栓法造成局灶性脑缺血再灌注模型,应用免疫组织化学技术检测神经前体细胞的表达。主要观察指标:大脑侧室管膜、室管膜下区皮质和纹状体Nestin阳性细胞数。结果:脑缺血再灌注后,牛珀至宝微丸组缺血侧室管膜、室管膜下区、皮质和纹状体Nestin阳性细胞数显著多于对照组(P<0.05),且神经病学评分明显低于对照组(P<0.05)。结论:牛珀至宝微丸能持续上调脑缺血再灌注后Nestin的表达,这可能是其治疗缺血性脑血管病的机制之一。BACKGROUND: The adult individual hippocampus and subependyma cells are able to divide and produce neoformative neurons and separate cell population which are characterized by continuous division and proliferation and multiple differential potential energyalso named as neural stem cells. But the separation and purification are very difficult, or the separation process is apt to cause injury of neural cell and affect its activity to result in death.Therefore,it is significant essentially to develop Chinese herbs so as to induce the proliferation of neural stem cells in the body in clinic. OBJECTIVE:To observe the effect of niupo zhibao weiwan(NZW) on neural precursor after focal cerebral ischemic reperfusion,and probe into its mechanism in the treatment of ischemic cerebral vascular disease. DESIGN:Randomized, controlled, single blind trial design. SETTING and MATERIALS:The experiment was performed in the Laboratory of Neural Anatomy of Basic Institute in Guanzhou University of Traditional Chinese Medicine.Twenty four purebred SD rats were employed. INTERVENTION: Linear embolism in cerebral arteries was adopted to prepare the model of focal cerebral ischemia and the immunohistochemical technique was provided to determine the expression of neural precursor cells. MAIN OUTCOME MEASURES:Nestin positive cell counts in paraependyma, subependyma, cortex and striate body of cerebrum. RESULTS: After cerebral ischemic reperfusion,compared with the control group, in NZW group,Nestin positive cell counts in paraependyma, subependyma,cortex and striate body were significantly more and the neurology evaluation was remarkably lower(P< 0.05). CONCLUSION:NZW increases Nestin expression after cerebral ischemic reperfusion persistently,which is probably one of the mechanisms in the treatment of ischemic cerebral vascular diseases.

关 键 词：牛珀至宝微丸 局灶性脑缺血再灌注 神经前体细胞 室管膜下区 治疗 对照组 缺血性脑血管病 对局 学院 结论 

分 类 号：R743[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]