脑胶质瘤浸润淋巴细胞的分离、培养与表型及体外抗肿瘤活性测定  

Isolation,Culture,Phenotype and in Vitro Antitumor Cytolytic Activity or Glioma-infiltrating Lymphocytes of Brain

作　　者：康德智[1] 张国安[1] 刘小朋[1] 陈锦峰[1] 陈紫榕[1] 

机构地区：[1]福建医学院附属第一医院神经外科,空军福州医院临床免疫研究室

出　　处：《福建医学院学报》1994年第2期120-123,共4页

摘　　要：经重组白细胞介素２（ｒＩＬ－２）和胸腺因子Ｄ（ＴＦＤ）培养前后的人脑胶质瘤浸润淋巴细胞（ＧＩＬ）的表型及抗自体瘤细胞活性变化。（１）ＧＩＬ可被分离，培养后可增值；（２）新鲜ＧＩＬ主要为Ｔ淋巴细胞，其中ＣＤ＋８细胞多于ＣＤ４细胞，随培养时间延长呈相反变化；（３）新鲜ＧＩＬ活性很弱，培养后很强；（４）ＴＦＤ可能只有增强ｒＩＬ－２的激活作用。提示ＧＩＬ可望作为理想的效应细胞用于脑胶质瘤的治疗。We report the changes of phenotype and antitumor cytolytic activity of glioma-infiltrating lymphocytes(GIL)before and after recombinant interleukin-2(rIL-2)and thymic factor D(TFD).The results indicated that(a)GIL could be isolated from the human gliomas and proliferate after initiated in vitro;(b)most of fresh GIL were T lymphocytes,in which CD8 cells were more than CD4 cells, while the former became declining and the latter growing with time prolonged in culture;(c)killing activity of fresh GIL was weak,but that of initiat ed GIL was strong;(d)TFD could potentiate the initiated roles of rIL-2.Therefroe,it is reasonable to believe that GIL will be used as an ideal effector cells in the treatment of human brain gliomas.

关 键 词：脑胶质瘤 淋巴细胞 表型 RIL-2 体外抗肿瘤活性 浸润 治疗 培养 新鲜 效应细胞 

分 类 号：R739.41[医药卫生—肿瘤] R392[医药卫生—临床医学]