性激素及DMH对大鼠结肠粘膜细胞增殖活力的影响  

INFLUENCE OF SEX HORMONES AND 1,2-DIMETHYLHYDRAZINE ON PROLIFERATIVE ACTIVITY OF COLONIC EPITHELIAL CELLS IN SPRAGUE-DAWLEY RATS

作　　者：黄培林[1] 张瑞英[1] 刘东风[1] 张晓明[1] 

机构地区：[1]南京铁道医学院病理教研室

出　　处：《南京铁道医学院学报》1994年第2期74-78,111,共6页Journal of Nanjing Railway Medical College

摘　　要：应用二甲肼（ＤＭＨ）按４ｍｇ／ｋｇ体重、４０ｍｇ／ｋｇ体重２个剂量，同时给予去势、雌激素或雄激素，分别观察性激素及高、低剂量致癌物对Ｓｐｒａｇｕｅ－Ｄａｗｌｅｙ大鼠结肠粘膜细胞增殖活力的影响。结果显示性激素对肠粘膜细胞的增殖具有一定的影响，高、低剂量ＤＭＨ均可使肠粘膜细胞增殖活跃、旺盛甚至发生癌变，且存在剂量——效应关系。初步探讨了ＤＭＨ诱癌过程可能是一个增殖－异常增殖－肿瘤过程，认为结肠粘膜隐窝细胞增殖活力的明显增强时，应警惕有癌前病变甚至结肠癌的发生。wenty-seven Sprague-Dawley castrated rats were injected with 1,2-dimethylhydrazine(DMH)at the doses of 4 and 40 mg/kg body weight,at the same time, withdistilled water, estrogen or androgen,and nine rats served as controls. The cell proliferatlve ac-tivity of colon epithelial cells in rats was studied by H-thymidine(H-TdR)labeled radio-au-tographic analysis. The results indicated that sex hormones affected colonic epithelial cells proliferation,and that both high and low doses of DMH may promote cell proliferation,or even car-cinogenesis,and there was a positive dose-effect correlation of the proliferative activity in coloncrypt cells with the doses of DMH used.The findings suggest that colorectal carcinogenesis induced by DMH may involove proliferation-atypical hyperplasia-carcinogenesis;Therefore,hy-peractive crypts would have a selective advantage over other glaiids allowing earlier expression ofpreneoplastic changes aideveii coloi1ic carcinoma.

关 键 词：结肠粘膜 细胞增殖 性激素 大鼠 肿瘤 影响 剂量 活力 体重 雄激素 

分 类 号：R735[医药卫生—肿瘤] R574.62[医药卫生—临床医学]