高效液相色谱法检测动物性食品中甲苯咪唑残留  

Determination of mebendazole residue in eel muscles by HPLC

作　　者：杨方[1] 李耀平[1] 李小晶[1] 

机构地区：[1]福建出入境检验检疫局,福州350001

出　　处：《中国卫生检验杂志》2004年第5期545-546,共2页Chinese Journal of Health Laboratory Technology

摘　　要：建立鳗鱼体内甲苯咪唑残留的分析方法。采用乙酸乙酯/氨水作为提取溶剂,提取液挥干后将剩余的油状物溶于正已烷中,经NH2固相萃取小柱净化后,采用高效液相色谱进行分析。方法在1～500ng之间呈线性相关,回归方程r=6832.4x-1810.3,相关系数r=0.9999,检出限为0.4ng。对于0.025mg/kg、0.050mg/kg、0.10mg/kg三个添加水平,平均回收率为79.6%～88.1%,相对标准偏差为4.84%-8.36%。A method of detecting mebendazole residue in eel muscles was established. Mebendazole residue was extracted from eel muscle with ethyl acetate/ammonia,followed by sample cleanup with NH2 solid- phase extraction cartridge, the eluate was quantitated by liquid chromatography with DAD detector. The linear range is 1-500ng and the detection limit was 0.4ng.For spiked eel samples, the recoveries were between 79.6 -88.1 % , the relative standard deviations were between 4.84% - 8.36%. The method was useful for the determination of mebendazole residue in eel muscles.

关 键 词：甲苯咪唑 高效液相色谱法 体内 残留 提取液 提取溶剂 正已烷 动物性食品 添加水平 水作 

分 类 号：R532[医药卫生—内科学] S859.84[医药卫生—临床医学]