核基质附着区与转基因表达  

Matrix Attachment Regions and Transgene Expression

作　　者：黄慧珍[1] 陈士云[1] 吉万全[2] 王瑶[1] 

机构地区：[1]中国科学院武汉病毒研究所,武汉430071 [2]西北农林科技大学农学院,杨凌712100

出　　处：《中国生物工程杂志》2004年第9期2-6,共5页China Biotechnology

基　　金：国家自然科学基金资助项目 ( 3 0 2 0 0 2 2 2 )

摘　　要：核基质附着区 (matrixattachmentregions,MARs)是与核基质 (或核骨架 )特异结合的DNA序列 ,属于非编码序列 ,富含AT。通过与核基质的结合 ,它能使染色质形成独立的环状结构 ,调控基因的转录和表达 ,减少由于位置效应引起的转基因沉默。MARs在提高转基因表达水平、消除转基因个体间表达水平的差异、抑制转基因沉默等方面起着重要的作用。Matrix attachment regions (MARs) are DNA sequences that bind to the nuclear matrix,which tend to be noncoding sequences and AT rich.MARs can regulate gene transcription and expression,and suppress position dependent transgene silencing by forming chromatin into independent loop domain through their anchoring to the nuclear matrix.Studies have shown that MARs play very important roles in enhancing the level of transgene expression,decreasing the variability of transgene expression and suppressing transgene silencing.The structural and functional features of MARs and their application in genetic engineering of plants and animals were summarized.;

关 键 词：核基质 转基因表达 MAR 附着 表达水平 调控基因 DNA序列 转基因沉默 非编码序列 位置效应 

分 类 号：Q786[生物学—分子生物学] R394[医药卫生—医学遗传学]