原发性胆汁性肝硬化外周血T细胞亚群及细胞因子的检测  

Detection of serum cytokines and T lymphocyte subsets in peripheral blood in chinese patients with primary biliary cirrhosis

作　　者：何文革[1] 孙永涛[1] 邹爱民[2] 

机构地区：[1]第四军医大学唐都医院全军感染病诊疗中心,西安710038 [2]唐都医院中心实验室

出　　处：《胃肠病学和肝病学杂志》2004年第5期511-513,共3页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的　探讨中国人原发性胆汁性肝硬化患者的T细胞亚群及细胞因子存在状况及意义。方法　选未经特殊治疗的原发性胆汁性肝硬化 (PBC) 2 5例 ,性别、年龄匹配的健康人 2 0例作为对照组。以双抗体夹心ELISA法检测外周血细胞因子IL_2、IFN_γ、IL_4、IL_10、TNF_α水平。以S_P法检测外周血T淋巴细胞亚群。结果　与对照组比较 ,PBC患者CD+ 4 T细胞升高 ,CD+ 8T细胞下降 ,CD+ 4 /CD+ 8比值上升 (P <0 0 1)。PBC组IL_2、IFN_γ、TNF_α明显升高 (P <0 0 1) ,IL_10轻度上升 (P <0 0 5 ) ,IL_4水平与对照组比较无差异 (P >0 0 5 )。结论　PBC外周血CD+ 4 T细胞和Th1细胞因子占优势 。Objective To investigate significance of serum cytokines and T lymphocyte subsets in peripheral blood in chinese patients with primary biliary cirrhosis.Methods ELISA and S_P assay were used to detect the levels of IL_2,IFN_γ,IL_4,IL_10 and TNF_α in serum and T lymphocyte subsets in peripheral blood in 25 patients with primary biliary cirrhosis and 20 healthy persons with age and gender matched.Results Compared to control group,It was found in PBC (1)the number of CD4^+ T lymphocyte,CD^+_4/CD^+_8 ratio and the levels of IL_2,IFN_γ and TNF_α were significantly increased(P<0.01),(2) The level of IL_10 was slightly increased(P<0.05),(3)CD^+_8 T cell was significantly decreased(P<0.01),(4) There was no difference at the level of IL_4 between PBC and healthly person(P>0.05).Conclusion CD^+_4 T lymphocyte and Th1 cytokines are predominant in peripheral blood in chinese patients with primary biliary cirrhosis.It is suggested that they may play an important role in the pathogenesis of PBC.

关 键 词：PBC 原发性胆汁性肝硬化 对照组 亚群 细胞因子 CD4^+T细胞 外周血T细胞 水平 IL-10 中国人 

分 类 号：R575[医药卫生—消化系统] R392[医药卫生—内科学]