脑复聪对脑缺血小鼠脑内SOD和GSH-Px的影响  

Effect of Naofucong Grain’s on Cerebral SOD and GSH-Px in Cerebral Ischemic Mice

作　　者：张孟仁[1] 郭赛珊[1] 徐惠媛[1] 左萍萍[2] 杨楠[2] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院,北京100730 [2]中国医学科学院中国协和医科大学基础医学研究所

出　　处：《中国预防医学杂志》2004年第5期324-326,共3页Chinese Preventive Medicine

基　　金：国家科技部资助课题 (1 999年 )

摘　　要：目的　探讨自拟中药方———脑复聪颗粒对脑缺血小鼠记忆能力及大脑皮层超氧化物歧化酶 (SOD)和谷胱甘肽过氧化物酶 (GSH -Px)活性的影响。方法　采用双颈总动脉闭塞 -缺血 -再灌术造成学习记忆障碍小鼠模型。小鼠记忆能力以Morris圆形水迷宫的潜伏期作为指标。结果　小鼠脑缺血后 7d潜伏期〔(82 3± 3 2 0 )s〕比假手术组〔 (3 5 3± 3 7 9)s〕延长 (P <0 0 1) ;脑复聪组〔 (3 0 6± 3 1 3 )s〕恢复正常 (P <0 0 1)。脑缺血小鼠大脑皮层SOD和GSH -Px活性 (68 5 9± 2 5 5 ,10 88± 1 0 1)比假手术组 (79 68± 5 69,13 0 9± 2 5 3 )降低 ,脑复聪及当归芍药散治疗组提高到正常水平 (77 5 6± 4 88,13 3 5± 3 3 8;75 77± 6 88,13 3 2± 2 69) (P <0 0 5～ 0 0 1)。结论　脑复聪颗粒对脑缺血所致的智能障碍有保护作用 ,其机制可能与提高SOD和GSH -Px活性、对抗自由基损伤有关。Objective To investigate the effects of Naofucong grain on memory and cerebral superoxide Dismutase(SOD)、glutathione perioxidase(GSH-Px)activities in cerebral ischemic mice. Methods Mice models of learning/memory impairment were induced by bilateral ligation of the internal carotid arteries.Memory abilities were assessed by measurements of latent period in the Morris Water Maze. Results On the 7th day after the operation of bilateral ligation,latent periods〔(82.3±32.0)s〕were longer than those of pseudo operated mice〔(35.3±37.9)s〕(P<0.01);administration with Naofucong grain ameliorated those changes〔(30.6±31.3)s〕(P<0.01).The SOD and GSH-Px activities in brain cortex(68.59±2.55,10.88±1.01)were lower than those in pseudo operated mice(79.68±5.69,13.09±2.53),administration with Naofucong grain or Dangguishaoyao powder showed amelioration to normal levels(77.56±4.88,13.35±3.38;75.77±6.88,13.32±2.69)(P<0.05～0.01) Conclusion Naofucong grain possesses protective function on intellectual deficits in cerebral ischemic mice,which may be related to the enhancement of the cerebral SOD、GSH-Px activities.

关 键 词：脑复聪 脑缺血 小鼠 SOD GSH-PX 脑内 正常 记忆能力 结论 抗自由基损伤 

分 类 号：R743.3[医药卫生—神经病学与精神病学]